Survival of skin allografts is prolonged in mice with a dominant-negative H-Ras
Survival of skin allografts is prolonged in mice with a dominant-negative H-Ras
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DOI：
10.1016/j.trim.2007.10.009
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发表时间：
2008-02-01
影响因子：
1.5
通讯作者：
Li, Xiao-Kang
中科院分区：
文献类型：
--
作者：
Funeshima-Fuji, Naoko;Fujino, Masayuki;Li, Xiao-Kang
Ras是一种鸟嘌呤核苷酸结合蛋白，在调节T细胞增殖中起主要作用。探讨T细胞受体下游信号转导通路之一Ras/丝裂原活化蛋白激酶通路在同种异体抗原应答中的作用机制，我们在主要组织相容性复合物（MHC）不相容株BALB/c（H-2K（d））（供体）和T细胞特异性H-Ras显性阴性（dnRas）转基因（tg）C57 BL/6（H-2K（B））中进行全层皮肤移植。（受体）雄性小鼠。还进行了体外和体内dnRas tg小鼠T细胞增殖和细胞毒性T淋巴细胞（CTL）活性测定。对照B6/野生型（wt）小鼠同种异体移植物的中位移植物存活时间为7天。相反，dnRas tg小鼠组表现出显著的（p
Ras is a guanine nucleotide-binding protein that plays a major role in regulating the proliferation of T cells. To investigate the mechanism of the Ras/mitrogen-activated protein kinase pathway, one of the downstream signal-transduction pathways of T-cell receptors, in the response to alloantigen, we performed full-thickness skin grafting in the major histocompatibility complex (MHC) incompatible strain BALB/c (H-2K(d)) (donor) and T-cell-specific H-Ras dominant-negative (dnRas) transgenic (tg) C57BL/6 (H-2K(b)) (recipient) male mice. In vitro and in vivo dnRas tg mouse T-cell proliferation and cytotoxic T lymphocyte (CTL) activity assay were also performed. The median graft survival time in control B6/wild type (wt) mouse allografts was seven days. Conversely, the dnRas tg mouse group exhibited a significant (p