In vivo gene transfer into the adult honeybee brain by using electroporation
In vivo gene transfer into the adult honeybee brain by using electroporation
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DOI：
10.1016/j.bbrc.2004.03.178
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发表时间：
2004-05-21
影响因子：
3.1
通讯作者：
Kubo, T
中科院分区：
文献类型：
--
作者：
Kunieda, T;Kubo, T
蜜蜂，意大利蜜蜂，是一种社会性昆虫，它们表现出各种各样精致的社会行为来维持群体活动。为了在分子水平上阐明这些社会行为和神经系统中的基因功能，我们开发了一种通过电穿孔在活体蜜蜂成年大脑中进行基因转移的体内方法。当用该系统将表达绿色荧光蛋白的质粒转移到脑中时，在阳极位置附近观察到绿色荧光。通过逆转录-聚合酶链反应和免疫印迹分析证实了转染基因在转录和翻译水平上的表达。该系统将有助于分析基因功能和神经系统中基因网络的调控机制，并为阐明这些基因与蜜蜂复杂行为之间的关系提供线索。(C)2004年爱思唯尔公司All rights reserved.
The honeybee, Apis mellifera L., is a social insect and they show wide variety of exquisite social behaviors to maintain colony activity. To enable the elucidation of those social behaviors at a molecular level and gene function in the nervous system, we developed an in vivo method to perform gene transfer in the adult brain of living honeybee by electroporation. When green fluorescent protein-expressing plasmid was transferred to the brain with this system, green fluorescence was observed near the anode location. The expression of transfected genes was confirmed at both transcriptional and translational levels by reverse transcription-polymerase chain reaction and immunoblot analyses. This system will facilitate the analysis of gene function and the regulatory mechanisms of gene networks in the nervous system and provide clues to clarify the relation between those genes and the complex behaviors of the honeybee. (C) 2004 Elsevier Inc. All rights reserved.