Persistent enterovirus infection in culture-negative meningoencephalitis: demonstration by enzymatic RNA amplification.
Persistent enterovirus infection in culture-negative meningoencephalitis: demonstration by enzymatic RNA amplification.
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培养阴性脑膜脑炎中的持续肠道病毒感染：通过酶 RNA 扩增证实。
DOI：
10.1093/infdis/161.4.787
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Wasserman,RL
中科院分区：
文献类型：
--
作者：
Rotbart,HA;Kinsella,JP;Wasserman,RL
肠道病毒感染引起的慢性脑膜脑炎可发生在抗体缺乏的患者中。尽管常规病毒培养和常规核酸杂交呈阴性，但改进的聚合酶链反应技术证明该患者存在持续的埃可病毒11感染。尽管埃可病毒 11 的序列尚未确定，但肠道病毒之间的基因组保守性是显着的，允许使用源自已完全测序的肠道病毒血清型的引物对和探针检测埃可病毒 11。这项研究为阴性病毒培养物中肠道病毒感染的持续存在提供了第一个明确的证据。
Chronic meningoencephalitis due to enterovirus infection can occur in patients with antibody deficiencies. A modified polymerase chain reaction technique demonstrated persistent echovirus 11 infection in such a patient, despite negative routine viral cultures and negative routine nucleic acid hybridization. Although the sequence of echovirus 11 has not yet been determined, genomic conservation among the enteroviruses is significant, permitting detection of echovirus 11 with a primer pair and probe derived from enterovirus serotypes that have been fully sequenced. This study provides the first definitive evidence for the persistence of enterovirus infection with negative viral cultures.