Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles
Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles
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DOI：
10.1002/jps.1143.abs
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发表时间：
2001-12-01
影响因子：
3.8
通讯作者：
Albrecht, RM
中科院分区：
文献类型：
--
作者：
Hillyer, JF;Albrecht, RM
胃肠道吸收的微米和纳米粒子一直是最近的努力，以开发有效的载体，提高口服药物和疫苗的主题。在这里，我们使用相关的仪器中子活化分析和电子显微镜定量和定性研究胃肠道摄取和随后的组织/器官分布的4，10，28和58 nm直径的金属胶体金颗粒口服给药后，小鼠。在我们的定量研究中，我们发现胶体金的摄取取决于颗粒大小：较小的颗粒更容易穿过胃肠道。电子显微镜研究表明，颗粒摄取发生在小肠的persorption通过单一的，降解的肠上皮细胞的过程中被挤出的绒毛。据我们所知，这是第一份报告，在超微结构水平上，通过挤压肠细胞产生的孔，通过persorption胃肠道摄取颗粒。(C)2001 Wiley-Liss，Inc.和美国制药协会。
The gastrointestinal uptake of micro- and nanoparticles has been the subject of recent efforts to develop effective carriers that enhance the oral uptake of drugs and vaccines. Here, we used correlative instrumental neutron activation analysis and electron microscopy to quantitatively and qualitatively study the gastrointestinal uptake and subsequent tissue/organ distribution of 4, 10, 28, and 58 nm diameter metallic colloidal gold particles following oral administration to mice. In our quantitative studies we found that colloidal gold uptake is dependent on particle size: smaller particles cross the gastrointestinal tract more readily. Electron microscopic studies showed that particle uptake occurred in the small intestine by persorption through single, degrading enterocytes in the process of being extruded from a villus. To our knowledge this is the first report, at the ultrastructural level, of gastrointestinal uptake of particulates by persorption through holes created by extruding enterocytes. (C) 2001 Wiley-Liss, Inc. and the American Pharmaceutical Association.