Stimulation of adult neural stem cells with a novel glycolipid biosurfactant.
Stimulation of adult neural stem cells with a novel glycolipid biosurfactant.
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用新型糖脂生物表面活性剂刺激成体神经干细胞。
DOI：
10.1007/s13760-013-0232-4
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发表时间：
2013
影响因子：
2.7
通讯作者：
Kippin,TodE
中科院分区：
文献类型：
--
作者：
Stipcevic,Tamara;Knight,ChristopherP;Kippin,TodE
糖脂是两亲性分子，其在皮肤和脑中的细胞膜上高度表达，在那里它们介导几个关键的细胞过程。神经干细胞是一种未分化的、增殖性的、多潜能的细胞，具有广泛的自我更新能力，对脑损伤有反应。二鼠李糖脂：α-l-吡喃鼠李糖基-（1-2）α-l-吡喃鼠李糖基-3-羟基癸酰基-3-羟基癸酸，也称为二鼠李糖脂BAC-3，是从细菌铜绿假单胞菌中分离的糖脂。在先前的研究中，二鼠李糖脂增强了真皮组织愈合和再生。本研究提供了第一次评估二-鼠李糖脂，糖脂生物表面活性剂一般，对神经系统。与对照组相比，从成年小鼠侧脑室分离并在含有0.5和1 μg/ml二鼠李糖脂的生长因子的成分确定的培养基中培养的神经干细胞的处理增加了神经球的数量（分别为2.7和2.8倍），并且即使在不存在二鼠李糖脂的情况下传代后，这种效果仍然存在。此外，在补充有胎牛血清且不含生长因子的限定培养基中，用50和100 μg/ml二鼠李糖脂处理的神经干细胞表现出细胞活力增加，表明二鼠李糖脂和血清组分在神经干细胞和神经祖细胞的调节中存在相互作用。脑室内给予300和120 ng/天的二鼠李糖脂可增加成年小鼠前侧脑室产生的神经球数量（分别为1.3倍和1.63倍）。这些结果表明，二-鼠李糖脂刺激神经干细胞的增殖，并增加其内源性库，这可能在管理神经退行性或神经精神疾病和促进损伤后的神经组织再生方面具有治疗潜力。
Glycolipids are amphipathic molecules which are highly expressed on cell membranes in skin and brain where they mediate several key cellular processes. Neural stem cells are defined as undifferentiated, proliferative, multipotential cells with extensive self-renewal and are responsive to brain injury. Di-rhamnolipid: α-l-rhamnopyranosyl-(1-2)α-l-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid, also referred to as di-rhamnolipid BAC-3, is a glycolipid isolated from the bacteriaPseudomonas aeruginosa. In the previous studies, di-rhamnolipid enhanced dermal tissue healing and regeneration. The present study provides the first assessment of di-rhamnolipid, and glycolipid biosurfactants in general, on the nervous system. Treatment of neural stem cells isolated from the lateral ventricle of adult mice and cultured in defined media containing growth factors at 0.5 and 1 μg/ml of di-rhamnolipid increased the number of neurospheres (2.7- and 2.8-fold, respectively) compared to controls and this effect remained even after passaging in the absence of di-rhamnolipid. In addition, neural stem cells treated with di-rhamnolipid at 50 and 100 μg/ml in defined media supplemented with fetal calf serum and without growth factors exhibited increased cell viability, indicating an interaction between di-rhamnolipid and serum components in the regulation of neural stem cells and neuroprogenitors. Intracerebroventricular administration of di-rhamnolipid at 300 and 120 ng/day increased the number of neurospheres (1.3- and 1.63-fold, respectively) that could be derived from the anterior lateral ventricles of adult mice. These results indicate that di-rhamnolipid stimulates proliferation of neural stem cells and increases their endogenous pools which may have therapeutic potential in managing neurodegenerative or neuropsychiatric disorders and promoting nervous tissue regeneration following injury.