A Comparative Tissue-specific Metabolite Analysis and Determination of Protodioscin Content in Asparagus Species used in Traditional Chinese Medicine and Ayurveda by use of Laser Microdissection, UHPLC-QTOF/MS and LC-MS/MS
A Comparative Tissue-specific Metabolite Analysis and Determination of Protodioscin Content in Asparagus Species used in Traditional Chinese Medicine and Ayurveda by use of Laser Microdissection, UHPLC-QTOF/MS and LC-MS/MS
复制标题
DOI：
10.1002/pca.2522
复制
发表时间：
2014-11-01
影响因子：
3.3
通讯作者：
Zhao, Zhongzhen
中科院分区：
文献类型：
--
作者：
Jaiswal, Yogini;Liang, Zhitao;Zhao, Zhongzhen
前言芦笋在中医学和阿育吠陀中备受推崇，是全球草药市场上最重要的药材之一。不同物种的比较代谢物图谱将有助于确定它们的相似性，并确定它们用作彼此替代品的有效性。激光显微解剖(LMD)有助于识别特定组织中的代谢物，从而有助于探索靶组织中的代谢途径。默尔。和总状花序芦笋。方法采用UHPLCQTOF/MS和LC-MS/MS对激光切割的组织进行代谢分析，测定原山药皂苷的含量，并参照国际协调人体用药品注册技术要求会议的指南进行验证。结果代谢分析结果表明，在皮质、维管束和髓等组织中，原山药皂苷的含量最高，尤其是类固醇皂苷类。结论本研究为利用LMD、UHPLCQTOF/MS和LC-MS/MS测定芦笋的代谢物组成和含量提供了一种合适的方法，表明印度芦笋和中国芦笋在不同的治疗和药物应用中可以相互替代。版权所有(C)2014 John Wiley&Sons，Ltd.。本研究比较了天冬的组织特异性代谢物和原薯蓣皂苷的含量。默尔。和总状花序A.racemosus Wild.激光显微解剖组织，UHPLC-QTOF/MS和LC-MS/MS分析代谢物。结果表明，与其他解剖组织相比，黄连的绒毛组织中含有最多的甾体成分，而黄连中的原薯蓣皂苷含量较高。所选芦笋种块茎代谢产物的相似性表明，它们可以在质量上相互替代。
IntroductionAsparagus is esteemed in Traditional Chinese Medicine and Ayurveda, and it is commercially one of the most important drugs in the global herbal market. Comparative metabolite profiling of different species would help in determining the similarities and ascertain their validity for being used as substitutes for each other. Laser microdissection (LMD) facilitates identification of metabolites in specific tissues, and thus it can aid in exploration of metabolic pathways in target tissues.ObjectiveTo compare tissue-specific metabolites and protodioscin content of Asparagus cochinchinensis (Lour.) Merr. and Asparagus racemosus Willd. used in China and India.MethodsMetabolite analysis of laser-dissected tissues was carried out using UHPLC-QTOF/MS and LC-MS/MS. The protodioscin contents were determined and the method was validated as per the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines.ResultsMetabolite analysis reveals that the velamen tissue, among other tissues such as cortex, vascular bundles and pith, contained maximum components, specifically those belonging to the steroidal saponin class. Although the metabolite profiles were similar, the content of protodioscin was found to be higher in Chinese than Indian species.ConclusionThe study provided a suitable methodology for metabolite profiling and protodioscin content determination of Asparagus by use of LMD, UHPLC-QTOF/MS and LC-MS/MS. The similarities in metabolite profiles indicate that Asparagus species from India and China can serve as substitute for each other in various therapeutic and pharmaceutical applications. Copyright (c) 2014 John Wiley & Sons, Ltd.The present study compares the tissue-specific metabolites and protodioscin content of Asparagus cochinchinensis (Lour.) Merr. and A. racemosus Willd. Tissues were Laser micro-dissected and UHPLC-QTOF/MS and LC-MS/MS were used for metabolite analysis. Results indicate that the velamen tissue contained maximum number of steroidal constituents compared to the other dissected tissues and A. cochinchinensis had higher content of protodioscin. Similarities in metabolite profiles of tubers of selected Asparagus species suggest that they can be qualitatively substituted for each other.