Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry.
Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry.
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使用靶向质谱法对小鼠和人类巨噬细胞分化过程中的甘油磷脂进行定量分析
DOI：
10.1038/s41598-017-00341-2
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发表时间：
2017-03-24
影响因子：
4.6
通讯作者：
Yu H
中科院分区：
文献类型：
--
作者：
Zhang C;Wang Y;Wang F;Wang Z;Lu Y;Xu Y;Wang K;Shen H;Yang P;Li S;Qin X;Yu H
巨噬细胞脂质代谢在先天性和适应性免疫反应中发挥着关键作用。以前的研究表明，这一过程在感染中起作用，并有助于糖尿病，动脉粥样硬化和其他免疫代谢疾病的发病机制。M1巨噬细胞或经典活化的巨噬细胞是防御细菌感染的关键参与者。M2巨噬细胞，或者活化的巨噬细胞，参与抗炎反应。使用多反应监测方法，我们确定了人类和小鼠巨噬细胞分化过程中脂质组成的变化。我们在哺乳动物巨噬细胞中检测到超过300种脂质分子，并且我们观察到在人类巨噬细胞极化期间甘油磷脂（GL）的组成从饱和和单不饱和到多不饱和的显著转变。此外，M2巨噬细胞显示出比M1巨噬细胞更高水平的溶血磷脂（lysoGL）。人和小鼠M2巨噬细胞中的lysoPI种类增加，表明它们可能参与M2巨噬细胞极化和抗炎过程。总的来说，这些结果表明，脂质可能在巨噬细胞的促炎和抗炎活性中发挥作用，并且可能是巨噬细胞活化状态的标志物。
Macrophage lipid metabolism plays a pivotal role in innate and adaptive immune responses. Previous studies have shown that this process plays a role in infections and contributes to the pathogenesis of diabetes, atherosclerosis, and other immunometabolic diseases. M1 macrophages, or classically activated macrophages, are key players in the defense against bacterial infections. M2 macrophages, or alternatively activated macrophages, are involved in anti-inflammatory responses. Using the multiple reaction monitoring method, we identified changes in lipid composition during the differentiation of human and murine macrophages. We detected over 300 lipid molecules in mammalian macrophages, and we observed a striking shift in the composition of glycerophospholipids (GLs) from saturated and monounsaturated to polyunsaturated during human macrophage polarization. Moreover, M2 macrophages showed a higher level of lysophospholipids (lysoGLs) than did M1 macrophages. The lysoPI species increased in human and mouse M2 macrophages, suggesting that they may be involved in M2 macrophage polarization and anti-inflammatory processes. Collectively, these results indicate that lipids may play a role in the pro- and anti-inflammatory activities of macrophages and may be markers of the macrophage activation state.