Plasma catecholamines determination using high pressure liquid chromatography and their roles in blood pressure regulation and experimental hypertension in rats.
Plasma catecholamines determination using high pressure liquid chromatography and their roles in blood pressure regulation and experimental hypertension in rats.
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使用高压液相色谱法测定血浆儿茶酚胺及其在大鼠血压调节和实验性高血压中的作用。
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发表时间：
1979
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通讯作者：
Y. Masuyama
中科院分区：
文献类型：
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作者：
T. Nishimura;I. Nishio;H. Ohtani;S. Jimbo;M. Kuchi;Y. Masuyama
血浆儿茶酚胺水平已被实验和临床用作交感神经活动的指标。我们用高压液相色谱法测定了大鼠血浆中的儿茶酚胺，以评估血浆中的儿茶酚胺作为交感神经活动的指标的意义及其在高血压中的作用。戊巴比妥麻醉降低血浆儿茶酚胺水平，尤其是血浆肾上腺素。钠负荷5周抑制血浆去甲肾上腺素，而呋塞米（1 mg/kg）的管理产生的血浆去甲肾上腺素的升高。实验性高血压，一肾和两肾型Goldblatt高血压和DOCA盐高血压，提高血浆去甲肾上腺素在急性和慢性阶段。升压剂量的血管紧张素II的输注通过反射机制抑制血浆去甲肾上腺素。SAR 1、Ile 8-血管紧张素II和SQ 14，225不能抑制出血引起的血浆儿茶酚胺升高。L-羟基多巴胺使实验性高血压大鼠和对照大鼠血浆中的儿茶酚胺升高。肾上腺去髓鞘后，血浆去甲肾上腺素减少6-羟基多巴胺的管理。急性循环血容量减少和血压下降引起血浆儿茶酚胺升高，尤其是血浆肾上腺素。在大鼠中，肾上腺髓质在血压调节中起重要作用。
Plasma catecholamine levels have been used experiemtally and clinically as the indices of the sympathetic nerve activity. We measured plasma catecholamines using high pressure liquid chromatography in rats to assess the significance of plasma catecholamines as an index of the sympathetic nerve activity and its role in hypertension. Pentobarbital anesthesia depressed plasma catecholamine levels, especially plasma adrenaline. Sodium loading for 5 weeks suppressed plasma noradrenaline, while administration of furosemide (1 mg/kg) produced the elevation of plasma noradrenaline. Experimental hypertension, one-kidney and two-kidney types of Goldblatt hypertension and DOCA-salt hypertension, raised plasma noradrenalines both in acute and chronic phases. The infusion of pressor doses of angiotensin II suppressed plasma noradrenaline by the reflex mechanism. Sar1, Ile8-angiotensin II and SQ 14,225 did not suppress plasma cathecholamine elevation due to hemorrhage. L-Hydroxyldopamine produced elevation of plasma catecholamines in experimental nypertension and controls in rats. After adrenal demedullation, plasma noradrenaline was decreased by the administration of 6-hydroxy-dopamine. Acute reduction of circulating blood volume and blood pressure fall produced the elevation of plasma catecholamine, especially plasma adrenaline. In rats, the adrenal medulla plays an important role in the regulation of blood pressure.