Immunohistochemical evaluation of heat shock proteins in normal and preinvasive lesions of the cervix
Immunohistochemical evaluation of heat shock proteins in normal and preinvasive lesions of the cervix
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DOI：
10.1016/j.canlet.2005.06.045
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发表时间：
2005-11-18
期刊：
影响因子：
9.7
通讯作者：
Muller, CY
中科院分区：
文献类型：
--
作者：
Castle, PE;Ashfaq, R;Muller, CY
热休克蛋白（HSPs）是一种具有多种功能的分子伴侣，与细胞稳态有关。首先，热休克蛋白的表达是对细胞应激的反应，也可能包括致癌作用。在宫颈癌的多步骤癌变过程中（正常-> HPV感染->宫颈癌前癌），表达模式尚未得到广泛评估。我们通过免疫组化染色（分级0-3）评估了HSP40、HSP60、HSP70和HSP90在正常组织（n30）、宫颈上皮内瘤变1级（CIN1）（与生产性HPV感染同音）（N= 32）和CIN3（宫颈癌前病变）（N=25）中的表达，并将结果与p16(INK4)a（致癌性HPV感染和CIN3的生物标志物）进行了比较。我们发现HSP40、HSP60和HSP70免疫染色随着病变严重程度的增加而增加，其模式与p16（INK4a）相似（P-Trend < 0.0005）。HSP90在不同病变级别的染色强度无差异（P-Trend=0.8）。HSP40和HSP70的弥漫免疫染色在CIN3中的组织模式与p16 （INK4a）相似；尽管观察到相似的组织模式，但HSP60免疫染色在细胞内比其他抗原出现更多的点状。我们得出结论，HSP40、HSP60和HSP70的表达与p16（INK4a）表达类似，随着CIN3的发展而上调。2005爱思唯尔爱尔兰有限公司版权所有。
Heat Shock Proteins (HSPs) are molecular chaperons with multiple functions relating to cellular homeostasis. Primary, HSPs are expressed in response to cellular stresses that may also include carcinogenesis. Patterns of expression have not been extensively evaluated in the multi-step carcinogenesis of cervical cancer (Normal -> HPV infection -> Cervical Precancer Cancer). We evaluated the expression of HSP40, HSP60, HSP70, and HSP90 in normal tissues (N 30), in cervical intraepithelial neoplasia grade 1 (CIN1)(synonymous with productive HPV infections) (N=32), and in CIN3 (cervical precancer)(N=25) by immunohistochemistry staining (graded 0-3) and compared the results to p16(INK4)a, a biomarker of oncogenic HPV infections and CIN3. We found strong patterns of increased HSP40, HSP60, and HSP70 immunostaining with increasing severity of the lesion in a manner similar to p16(INK4a) (P-Trend < 0.0005). No difference in staining intensity by grade of lesion was observed for HSP90 (P-Trend=0.8). Tissue patterns in CIN3 of diffuse immunostaining for HSP40 and HSP70 were analogous to those observed for p 16(INK4a); HSP60 immunostaining appeared more punctate within cells than for other antigens although similar tissue patterns were observed. We conclude that HSP40, HSP60, and HSP70 expressions are up-regulated in response to the development of CIN3 akin to p16(INK4a) expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.