Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons
Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons
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DOI：
10.1002/cne.21702
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发表时间：
2008-06-20
影响因子：
2.5
通讯作者：
Helfrich-Foerster, Charlotte
中科院分区：
文献类型：
--
作者：
Yoshii, Taishi;Todo, Takeshi;Helfrich-Foerster, Charlotte
隐色素（CRY）与许多生物体的昼夜节律钟密切相关。在果蝇Drosophila melanogaster中，CRY似乎与光感受和核心时钟装置有关。尽管CRY对果蝇的生物钟起着关键作用，但目前还不清楚CRY是否在每个生物钟细胞中表达。借助一种新的抗体和一种缺乏CRY的突变体，我们在这里展示了CRY在果蝇起搏神经元的特定亚群和复眼的感光细胞中表达。在起搏神经元中，光暗循环下的CRY水平和动力学相互之间存在很大差异。高振幅振荡只在三组时钟神经元中观察到，这表明这三组神经元对光有很强的接受能力。不同的CRY动力学可能解释了早期研究中在这三组中观察到的时钟蛋白振荡的相位差异。含有较低CRY水平或完全CRY阴性的神经元的分子钟仍然可以通过光同步，可能是通过细胞间与CRY阳性神经元的通讯以及通过外部光感受器。
Cryptochrome (CRY) is intimately associated with the circadian clock of many organisms. In the fruit fly Drosophila melanogaster, CRY seems to be involved in photoreception as well as in the core clockwork. In spite of the critical role of CRY for the clock of Drosophila, it was not quite clear whether CRY is expressed in every clock cell. With the help of a new antibody and a mutant that lacks CRY, we show here that CRY is expressed in specific subsets of Drosophila's pacemaker neurons and in the photoreceptor cells of the compound eyes. In the pacemaker neurons, CRY levels and kinetics under light-dark cycles are quite different from each other. High-amplitude oscillations are observed in only three groups of clock neurons, suggesting that these three groups are strongly receptive to light. The different CRY kinetics may account for phase differences in oscillations of the clock proteins observed in these three groups in earlier studies. The molecular clock of the neurons that contain lower CRY levels or are completely CRY negative can still be synchronized by light, probably via intercellular communication with the CRY-positive neurons as well as via external photoreceptors.