The use of Preyer's reflex in evaluation of hearing in mice.
The use of Preyer's reflex in evaluation of hearing in mice.
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DOI：
10.1080/00016480118142
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发表时间：
2001-07
影响因子：
1.4
通讯作者：
J. Jero;D. Coling;A. Lalwani
中科院分区：
文献类型：
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作者：
J. Jero;D. Coling;A. Lalwani
Preyer反射是对听觉刺激的惊吓反应，已被广泛用于啮齿动物和其他动物的听力评估。然而，令人惊讶的是，Preyer反射在听力评估中的敏感性和特异性尚未得到充分研究。本研究旨在探讨Preyer反射在小鼠听觉功能评价中的应用。本研究使用了46只具有不同听力损失的FVB背景的成年白化病小鼠。两种不同的方法引发普雷耶反射进行了测试：拍手和尖锐的金属声。当明显观察到动物全身快速运动时，认为反射为阳性。此后，小鼠进行了听觉脑干反应（ABR）测试与宽带点击。将Preyer反射的存在与否与相应的ABR阈值进行比较。研究的46只动物中有5只（11%）显示出阴性Preyer反射，而其余41只动物显示出阳性Preyer反射。两种不同的刺激引起普莱尔反射的能力没有差异。测试动物的点击诱发ABR阈值在8和136（平均50）dB声压级（SPL）之间变化。所有动物的Preyer反射均为阳性，ABR阈值≥ 81 dB SPL。Preyer反射对于识别实验小鼠的深度感音神经性听力损失是有效的，但是对于检测不太严重的听觉功能障碍是不敏感的。用于确定性听力评估和定义听力阈值。应使用客观的电声方法，如ABR。
Preyer's reflex, the elicitation of startle response to auditory stimuli, has been widely used for the evaluation of hearing in rodents and other animals. Surprisingly, however, the sensitivity and specificity of Preyer's reflex in the assessment of hearing has not been adequately studied. The aim of this study was to investigate the utility of Preyer's reflex in the evaluation of auditory function in mice. Forty-six adult albino mice on an FVB background with variable hearing loss were used for this study. Two different methods for eliciting a Preyer's reflex were tested: a handclap and a sharp metallic sound. The reflex was considered positive when a rapid movement of the whole body of the animal was clearly noticed. Thereafter, the mice underwent auditory brain stem response (ABR) testing with broadband clicks. The presence or absence of Preyer's reflex was compared with the corresponding ABR thresholds. Five of the 46 animals studied (11%) showed a negative Preyer's reflex, while the remaining 41 animals demonstrated a positive Preyer's reflex. There was no difference between the abilities of the two different stimuli to elicit a Preyer's reflex. The click-evoked ABR thresholds in the test animals varied between 8 and 136 (mean 50) dB sound pressure level (SPL). Preyer's reflex was positive in all animals with an ABR threshold of or = 81 dB SPL. Preyer's reflex is effective for identifying profound sensorineural hearing loss in experimental mice, but is insensitive for detecting less severe auditory dysfunction. For definitive hearing assessment, and for defining the hearing thresholds. objective electroacoustical methods such as ABR should be used.