Modeling physiological and pathological human neurogenesis in the dish.
Modeling physiological and pathological human neurogenesis in the dish.
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DOI：
10.3389/fnins.2014.00183
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发表时间：
2014
影响因子：
4.3
通讯作者：
Mazzara PG
中科院分区：
文献类型：
--
作者：
Broccoli V;Giannelli SG;Mazzara PG
在指导人胚胎和诱导多能干细胞（hPSC，旨在表达两类多能干细胞的缩写）的神经元分化方面的新进展已经促进了能够在其一些关键里程碑模拟早期神经发生和神经特化的培养系统的发展。hPSC衍生的神经花环可以被认为是发育中神经管的体外对应物，因为这两种结构具有几乎相同的结构和相关的功能特性。表观遗传刺激方法可以调节玫瑰花结神经祖细胞的身份，以产生真实的神经元亚型，以及全谱的神经嵴衍生物。诱导多能细胞衍生的神经组织自组织的内在能力已经随着能够将hPSC的神经元分化形成三维发育的组织化组织的创新性体外系统的出现而变得完全明显。然而，仍然存在必须完全解决的重大障碍，以便促进hPSC在建模中的使用（例如，迟发性疾病）或建立细胞替代的治疗策略。在这个方向上，已经建立了新的程序来促进hPSC衍生的神经元的成熟和功能。同时，加速体外分化细胞老化的新方法仍在开发中。基于hPSC的技术已经足够成熟，可以为早期和晚期神经发生提供重要而可靠的模型系统，这对于研究在此过程中发生的生理和病理事件非常有用。因此，充分利用这种细胞系统可以更好地了解塑造人类大脑结构的生理事件，以及研究人类疾病根源的病理机制的坚实平台。
New advances in directing the neuronal differentiation of human embryonic and induced pluripotent stem cells (hPSCs, abbreviation intended to convey both categories of pluripotent stem cells) have promoted the development of culture systems capable of modeling early neurogenesis and neural specification at some of their critical milestones. The hPSC-derived neural rosette can be considered the in vitro counterpart of the developing neural tube, since both structures share a virtually equivalent architecture and related functional properties. Epigenetic stimulation methods can modulate the identity of the rosette neural progenitors in order to generate authentic neuronal subtypes, as well as a full spectrum of neural crest derivatives. The intrinsic capacity of induced pluripotent cell-derived neural tissue to self-organize has become fully apparent with the emergence of innovative in vitro systems that are able to shape the neuronal differentiation of hPSCs into organized tissues that develop in three dimensions. However, significant hurdles remain that must be completely solved in order to facilitate the use of hPSCs in modeling (e.g., late-onset disorders) or in building therapeutic strategies for cell replacement. In this direction, new procedures have been established to promote the maturation and functionality of hPSC-derived neurons. Meanwhile, new methods to accelerate the aging of in vitro differentiating cells are still in development. hPSC-based technology has matured enough to offer a significant and reliable model system for early and late neurogenesis that could be extremely informative for the study of the physiological and pathological events that occur during this process. Thus, full exploitation of this cellular system can provide a better understanding of the physiological events that shape human brain structures, as well as a solid platform to investigate the pathological mechanisms at the root of human diseases.
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影响因子：
3.7
作者：
Kim, Dae-Sung;Lee, Dongjin R.;Kim, Dong-Wook
通讯作者：
Kim, Dong-Wook
影响因子：
64.8
作者：
Kriks, Sonja;Shim, Jae-Won;Piao, Jinghua;Ganat, Yosif M.;Wakeman, Dustin R.;Xie, Zhong;Carrillo-Reid, Luis;Auyeung, Gordon;Antonacci, Chris;Buch, Amanda;Yang, Lichuan;Beal, M. Flint;Surmeier, D. James;Kordower, Jeffrey H.;Tabar, Viviane;Studer, Lorenz
通讯作者：
Studer, Lorenz
影响因子：
64.8
作者：
Israel, Mason A.;Yuan, Shauna H.;Bardy, Cedric;Reyna, Sol M.;Mu, Yangling;Herrera, Cheryl;Hefferan, Michael P.;Van Gorp, Sebastiaan;Nazor, Kristopher L.;Boscolo, Francesca S.;Carson, Christian T.;Laurent, Louise C.;Marsala, Martin;Gage, Fred H.;Remes, Anne M.;Koo, Edward H.;Goldstein, Lawrence S. B.
通讯作者：
Goldstein, Lawrence S. B.
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.0910012107
发表时间：
2010-03-02
影响因子：
11.1
作者：
Hu, Bao-Yang;Weick, Jason P.;Zhang, Su-Chun
通讯作者：
Zhang, Su-Chun