Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines
Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines
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DOI：
10.1111/j.1439-0523.2007.01414.x
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发表时间：
2007-12-01
期刊：
影响因子：
2
通讯作者：
Snowdon, R. J.
中科院分区：
文献类型：
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作者：
Rygulla, W.;Friedt, W.;Snowdon, R. J.
再合成（RS）形式的油菜（甘蓝型油菜L.;基因组AACC，2n = 38）。（syn. campestris;基因组AA，2n 20）和甘蓝（Brassica oleracea L.）(CC，2n 18）是将抗真菌病原菌长孢轮枝菌（Verticillium longisporum）的抗性引入油菜基因库的潜在有用资源。大量的卷心菜（B. oleracea）种质已知对长孢葡萄球菌具有抗性;然而，B.甘蓝通常在种子中具有高水平的芥酸和芥子油苷，这降低了所得RS油菜品系用于油菜育种的适用性。在这项研究中，在甘蓝登记Kashirka 202（B.羽衣甘蓝capitata）和两个春甘蓝型油菜（B.拉帕湾olerifera），且不含芥酸。结果表明，与两个亲本和B相比，其中一个零芥酸RS油菜品系对长孢蠕孢菌具有高水平的抗性。napus对照。一些其他零芥酸RS线表现出的抗性水平与亲本加入。在大多数抗性RS品系中，抗性和零芥酸性状与可变的种子硫代葡萄糖苷含量相结合。不含芥酸、种子硫甙含量适中的RS油菜是利用B进行长孢镰刀菌数量抗性渐渗的理想基础材料。oleracea和B.将其转化为优良油菜育种系。
Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n 20) and Brassica oleracea L. (CC, 2n 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid-free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.