Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis
Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O-Acetylation in Arabidopsis
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DOI：
10.1104/pp.113.225193
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发表时间：
2013-11-01
期刊：
影响因子：
7.4
通讯作者：
Scheller, Henrik Vibe
中科院分区：
文献类型：
--
作者：
Manabe, Yuzuki;Verhertbruggen, Yves;Scheller, Henrik Vibe
还原壁乙酰化（RWA）蛋白参与植物细胞壁的乙酰化。以前，我们描述了一个单一的突变体，rwa 2，它具有约20%的低水平的O-乙酰化在叶细胞壁和没有明显的生长或发育表型。在这项研究中，我们产生了双重，三重，四重的功能丧失突变体的所有四个成员的RWA家族在拟南芥（拟南芥）。与rwa 2相反，三重和四重rwa突变体显示严重的生长表型，揭示了细胞壁乙酰化对植物生长发育的重要性。四重rwa突变体可以与在35 S启动子下表达的RWA 2蛋白完全互补，表明RWA蛋白的功能冗余。然而，木聚糖，（葡糖）甘露聚糖和木葡聚糖的乙酰化程度以及整体细胞壁乙酰化在三重突变体的不同组合中受到不同的影响，表明它们在底物偏好上的多样性。与野生型相比，rwa四重突变体的细胞壁乙酰化的整体程度降低了63%，并且对rwa四重突变体干细胞的组织化学分析表明，具有次生细胞壁的细胞类型的细胞分化存在缺陷。
The Reduced Wall Acetylation (RWA) proteins are involved in cell wall acetylation in plants. Previously, we described a single mutant, rwa2, which has about 20% lower level of O-acetylation in leaf cell walls and no obvious growth or developmental phenotype. In this study, we generated double, triple, and quadruple loss-of-function mutants of all four members of the RWA family in Arabidopsis (Arabidopsis thaliana). In contrast to rwa2, the triple and quadruple rwa mutants display severe growth phenotypes revealing the importance of wall acetylation for plant growth and development. The quadruple rwa mutant can be completely complemented with the RWA2 protein expressed under 35S promoter, indicating the functional redundancy of the RWA proteins. Nevertheless, the degree of acetylation of xylan, (gluco) mannan, and xyloglucan as well as overall cell wall acetylation is affected differently in different combinations of triple mutants, suggesting their diversity in substrate preference. The overall degree of wall acetylation in the rwa quadruple mutant was reduced by 63% compared with the wild type, and histochemical analysis of the rwa quadruple mutant stem indicates defects in cell differentiation of cell types with secondary cell walls.