Complete genome sequences of T5-related Escherichia coli bacteriophages DT57C and DT571/2 isolated from horse feces
Complete genome sequences of T5-related Escherichia coli bacteriophages DT57C and DT571/2 isolated from horse feces
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DOI：
10.1007/s00705-015-2582-0
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发表时间：
2015-12-01
影响因子：
2.7
通讯作者：
Letarov, Andrey V.
中科院分区：
文献类型：
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作者：
Golomidova, Alla K.;Kulikov, Eugene E.;Letarov, Andrey V.
我们报道了从同一马粪便标本中分离的两种大肠杆菌t5相关噬菌体DT57C和DT571/2的全基因组测序。这两个分离株96%的核苷酸序列相同，因此可以认为是t5样病毒属中同一新种的代表。由于最近的重组事件，这些噬菌体的ltfA基因发生了变化，这可能解释了所观察到的宿主范围差异，表明模块化机制对肠道生态系统中t5样噬菌体基因组的短期进化（或适应）做出了重大贡献。将我们的分离物与它们最近的亲缘体噬菌体T5进行比较，发现它们的基因组具有高度的总体一致性，几乎所有结构蛋白以及其他具有确定功能的蛋白的序列具有高度的保守性。同时，大量非结构蛋白基因的改变和非同源替换（大部分功能未知）以及尾纤维位点组织的显著差异支持DT57C和DT571/2形成与噬菌体T5明显不同的物种水平群的结论。
We report the complete genome sequencing of two Escherichia coli T5-related bacteriophages, DT57C and DT571/2, isolated from the same specimen of horse feces. These two isolates share 96 % nucleotide sequence identity and can thus be considered representatives of the same novel species within the genus T5likevirus. The observed variation in the ltfA gene of these phages, resulting from a recent recombination event, may explain the observed host-range differences, suggesting that a modular mechanism makes a significant contribution to the short-term evolution (or adaptation) of T5-like phage genomes in the intestinal ecosystem. Comparison of our isolates to their closest relative, coliphage T5, revealed high overall synteny of the genomes and high conservation of the sequences of almost all structural proteins as well as of the other proteins with identified functions. At the same time, numerous alterations and non-orthologous replacements of non-structural protein genes (mostly of those with unknown functions) as well as substantial differences in tail fiber locus organization support the conclusion that DT57C and DT571/2 form a species-level group clearly distinct from bacteriophage T5.