A SNP based linkage map of the turkey genome reveals multiple intrachromosomal rearrangements between the Turkey and Chicken genomes
A SNP based linkage map of the turkey genome reveals multiple intrachromosomal rearrangements between the Turkey and Chicken genomes
复制标题
DOI：
10.1186/1471-2164-11-647
复制
发表时间：
2010-11-20
期刊：
影响因子：
4.4
通讯作者：
Groenen, Martien A. M.
中科院分区：
文献类型：
--
作者：
Aslam, Muhammad L.;Bastiaansen, John W. M.;Groenen, Martien A. M.
背景资料：火鸡（Meleagris gallopavo）是一种重要的农业物种，是世界禽肉生产的第二大贡献者。火鸡的基因组资源为火鸡育种者提供了对商业火鸡品种进行遗传改良所需的工具，以获得重要的经济性状。火鸡的连锁图不仅对数量性状基因座的定位至关重要，而且可以作为将序列重叠群分配到特定染色体上的框架。鸡比较基因组学提供了深入了解基因组进化的机制，并有助于确定罕见的基因组事件，如基因组重排和重复/deletions.Results：18个全同胞家庭，包括1008（35 F1和973 F2）的鸟类，基因型为775个单核苷酸多态性（SNPs）。在775个SNPs中，570个是信息性的，用于构建火鸡的连锁图谱。最终的图谱包含28个连锁群中的531个标记。这些连锁群覆盖的总遗传距离为2，324厘摩（cM），最大的连锁群（81个位点）测量为326 cM。28个连锁群中所有标记的平均标记间距为4.6cM。火鸡和鸡的比较作图揭示了这两个species.Conclusion：我们的火鸡遗传图谱的531个标记之间的两个间，和57染色体内重排揭示了基因组长度为2,324 cM。我们的连锁图提供了一个改进以前发表的地图，因为更均匀的分布标记，因为地图是完全基于SNP标记，使更容易和更快的基因分型测定比微卫星标记在以前的连锁图。火鸡和鸡被证明具有高度保守的基因组结构，染色体间和染色体内重排的数量相对较少。
Background: The turkey (Meleagris gallopavo) is an important agricultural species that is the second largest contributor to the world's poultry meat production. The genomic resources of turkey provide turkey breeders with tools needed for the genetic improvement of commercial breeds of turkey for economically important traits. A linkage map of turkey is essential not only for the mapping of quantitative trait loci, but also as a framework to enable the assignment of sequence contigs to specific chromosomes. Comparative genomics with chicken provides insight into mechanisms of genome evolution and helps in identifying rare genomic events such as genomic rearrangements and duplications/deletions.Results: Eighteen full sib families, comprising 1008 (35 F1 and 973 F2) birds, were genotyped for 775 single nucleotide polymorphisms (SNPs). Of the 775 SNPs, 570 were informative and used to construct a linkage map in turkey. The final map contains 531 markers in 28 linkage groups. The total genetic distance covered by these linkage groups is 2,324 centimorgans (cM) with the largest linkage group (81 loci) measuring 326 cM. Average marker interval for all markers across the 28 linkage groups is 4.6 cM. Comparative mapping of turkey and chicken revealed two inter-, and 57 intrachromosomal rearrangements between these two species.Conclusion: Our turkey genetic map of 531 markers reveals a genome length of 2,324 cM. Our linkage map provides an improvement of previously published maps because of the more even distribution of the markers and because the map is completely based on SNP markers enabling easier and faster genotyping assays than the microsatellitemarkers used in previous linkage maps. Turkey and chicken are shown to have a highly conserved genomic structure with a relatively low number of inter-, and intrachromosomal rearrangements.