EFFECTS OF ENRICHED ENVORONMENT ON HISTOLOGY OF RAT CEREBRAL CORTEX
EFFECTS OF ENRICHED ENVORONMENT ON HISTOLOGY OF RAT CEREBRAL CORTEX
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DOI：
10.1002/cne.901230110
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发表时间：
1964-01-01
影响因子：
2.5
通讯作者：
KRECH, D
中科院分区：
文献类型：
--
作者：
DIAMOND, MC;ROSENZWEIG, MR;KRECH, D
研究发现，受到复杂环境刺激的大鼠大脑皮层的重量明显大于与其隔离的同窝出生大鼠。虽然很小（5%），但这种差异是如此一致，以至于需要进行组织学研究。使用雄性S1大鼠（加州大学心理学研究所）。在25日龄时，取成对的同窝仔，每对中的一只动物被置于以下组之一：第一组[环境复杂性和训练]（ECT）包含10只动物，一起圈养在一个大笼子里，并给予木制“玩具”和迷宫体验。第二个[隔离条件]（IC）涉及隔离在单个笼中的10只动物。实验完全重复。在105日龄时，将大鼠断头，并将脑置于10%福尔马林盐水中。将横向冷冻切片切成25微米。皮质下标志被用来识别从体感和视觉皮质中提取的切片。使用Windle改良Nissl染色的组织进行皮质深度测量和细胞分类计数。在用苏木精和伊红染色的邻近组织上，进行血管测量。用光学测微计测量从软脑膜表面到胼胝体的皮质深度，ECT组动物视觉区的皮质深度显著大于IC组动物。在躯体感觉区域，差异并不一致。神经元，神经胶质细胞和毛细血管较少，每个领域的动物经历丰富的条件。然而，ECT动物的每个视野中有更多的大血管。
The weight of the cerebral cortex has been found to be significantly greater in rats stimulated by a complex environment than in their isolated littermates. Though small (5%), this difference was so consistent as to require histological investigation. Male S1 rats (University of California Psychology Colony) were used. At 25 days of age, pairs of littermates were taken, one animal of each pair being placed in one of the following groups: The first [Environmental Complexity and Training] (ECT) contained ten animals, housed together in a large cage and given wooden "toys" and maze experience. The second [Isolated Condition ] (IC) involved 10 animals isolated in individual cages. The experiment was replicated completely. At 105 days of age the rats were decapitated, and the brains placed in 10% formolTsaline. Transverse, frozen sections were cut at 25 micra. Subcortical landmarks were used to identify sections taken from the somatosensory and visual cortex. Tissues stained with Windle''s modified Nissl stain were used for cortical depth measurements and differential cell counts. On adjacent tissues stained with hematoxylin and eosin, blood vessel measurements were taken. The depth of cortex, measured from the pial surface to the corpus cal-losum with an optical micrometer, was significantly greater for ECT than IC animals in the visual area. In the somatosensory area the differences were not as consistent. Neurons, glia and capillaries were less numerous per field in the animals experiencing the enriched condition. However, there were more large vessels per field in the ECT animals.