Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method
Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method
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DOI：
10.1111/j.1365-294x.2004.02207.x
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发表时间：
2004-07-01
影响因子：
4.9
通讯作者：
Vigilant, L
中科院分区：
文献类型：
--
作者：
Nsubuga, AM;Robbins, MM;Vigilant, L
使用非侵入性收集的样本（如粪便）进行遗传分析仍然是一个巨大的挑战，因为在获得可用DNA的程度上存在不可预测的变化。我们研究了多个变量对从野生山地大猩猩和黑猩猩粪便样本中提取的DNA数量的影响。收集时的温度与获得的DNA量之间存在较小的负相关性。将样品储存在RNAlater溶液中或使用硅胶珠干燥产生了类似的结果，但是使用一种新的方案获得了显著更高量的DNA，该方案将在乙醇中的短期储存与随后使用硅胶干燥相结合。
Genetic analysis using noninvasively collected samples such as faeces continues to pose a formidable challenge because of unpredictable variation in the extent to which usable DNA is obtained. We investigated the influence of multiple variables on the quantity of DNA extracted from faecal samples from wild mountain gorillas and chimpanzees. There was a small negative correlation between temperature at time of collection and the amount of DNA obtained. Storage of samples either in RNAlater solution or dried using silica gel beads produced similar results, but significantly higher amounts of DNA were obtained using a novel protocol that combines a short period of storage in ethanol with subsequent desiccation using silica.