Preferential rearrangement of V kappa 4 gene segments in pre-B cell lines.
Preferential rearrangement of V kappa 4 gene segments in pre-B cell lines.
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DOI：
10.1084/jem.172.2.559
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发表时间：
1990-08-01
期刊：
影响因子：
--
通讯作者：
Brodeur PH
中科院分区：
文献类型：
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作者：
Kalled SL;Brodeur PH
对小鼠成体骨髓来源的前B细胞系的体外V κ基因重排的检查显示，25个细胞系中有21个（84%）重排了V κ 4家族的成员。相反，从LPS刺激的成人脾细胞制备的两个V κ cDNA文库的分析表明，只有17%的IG κ cDNA含有属于V κ 4基因家族的序列。检查的前B细胞系的一半也共享8-kbp BamHI倒数产物（rp）。然而，这些rp不涉及相同的V κ基因，表明保守的BamHI位点存在于某些V κ基因的3'端。这种rp也很容易在正常成人脾细胞的DNA中检测到，这表明本研究中检测的体外重排代表了体内发生的kappa重排。我们认为，与成熟B细胞表达的不同的V κ库，涉及初始重排的IG κ基因座的种系V κ片段是高度限制性的，并且初始V κ 4重排之后可能是其他更随机的重组事件。
Examination of the in vitro V kappa gene rearrangements of murine adult bone marrow-derived pre-B cell lines reveals that 21 of 25 (84%) cell lines have rearranged a member of the V kappa 4 family. In contrast, analysis of two V kappa cDNA libraries prepared from LPS-stimulated adult spleen cells indicates that only 17% of the Ig kappa cDNAs contain sequences belonging to the V kappa 4 gene family. Half of the pre-B cell lines examined also share an 8-kbp BamHI reciprocal product (rp). However, these rp do not involve the same V kappa gene, indicating that conserved BamHI sites exist 3' of some V kappa genes. This rp is also readily detected in DNA from normal adult spleen cells, suggesting that the in vitro rearrangements examined in this study are representative of kappa rearrangements that occur in vivo. We suggest that, unlike the diverse V kappa repertoire expressed by mature B cells, the germline V kappa segments involved in initial rearrangements of the Ig kappa locus are highly restricted, and that an initial V kappa 4 rearrangement is probably followed by other, more random recombination events.