CLONING, SEQUENCING AND EXPRESSION OF THE HUMAN THYROTROPIN (TSH) RECEPTOR - EVIDENCE FOR BINDING OF AUTOANTIBODIES
CLONING, SEQUENCING AND EXPRESSION OF THE HUMAN THYROTROPIN (TSH) RECEPTOR - EVIDENCE FOR BINDING OF AUTOANTIBODIES
复制标题
DOI：
10.1016/0006-291x(89)92736-8
复制
发表时间：
1989-12-29
影响因子：
3.1
通讯作者：
VASSART, G
中科院分区：
文献类型：
--
作者：
LIBERT, F;LEFORT, A;VASSART, G
通过与狗促甲状腺素受体 (TSHr) cDNA 杂交筛选人甲状腺 cDNA 文库。对所得克隆进行测序，鉴定出编码 744 个氨基酸的成熟多肽的 2292 个残基开放阅读框，与狗 TSHr 具有 90.3% 的相似性。在人类甲状腺中鉴定出两个主要转录本（4.6 和 4.4 KB），这表明选择性剪接可以产生多种形式的人类 TSHr。 COS-7 细胞中编码序列的转染赋予这些细胞的膜制剂特异性结合 TSH 的能力。 TSH 结构完全被特发性粘液性水肿患者的免疫球蛋白制剂所取代。
A human thyroid cDNA library was screened by hybridization with a dog thyrotropin receptor (TSHr) cDNA. Sequencing of the resulting clones identified a 2292 residue open reading frame encoding a 744 amino acid mature polypeptide presenting 90.3% similarity with the dog TSHr. Two major transcripts (4.6 and 4.4 kilobases) were identified in the human thyroid which suggests that alternative splicing could generate multiple forms of human TSHr. Transfection of the coding sequence in COS-7 cells conferred to a membrane preparation of these cells the ability to bind specifically TSH. TSH building was completely displaced by immunoglobulin preparations from patients with idiopathic myxoedema.