Differential activation of the lectin and antimicrobial peptide genes in Sarcophaga peregrina (the flesh fly).
Differential activation of the lectin and antimicrobial peptide genes in Sarcophaga peregrina (the flesh fly).
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DOI：
10.1002/arch.20280
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发表时间：
2008-12
影响因子：
2.2
通讯作者：
Takahiro Tanji;H. Shiraishi;S. Natori;Ayako Ohashi-Kobayashi
中科院分区：
文献类型：
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作者：
Takahiro Tanji;H. Shiraishi;S. Natori;Ayako Ohashi-Kobayashi
麻蝇凝集素是一种免疫防御蛋白，在肉蝇（Sarcophagaperegrina）的免疫攻击后被转录诱导。到目前为止，我们已经发现，Sarcophaga凝集素基因在其启动子中具有多个NF-κ B结合基序。在这里，我们表明，从Sarcophaga-derived培养细胞，NIH-Sape-4，和幼虫的脂肪体的核提取物具有结合活性的凝集素基因启动子，其中一些是响应免疫刺激的多个kappaB基序。我们还从诱导剂、表达组织和消化道局部诱导的角度比较了凝集素基因和抗菌肽基因的表达谱。在每种情况下，凝集素基因被激活的方式与其他诱导防御基因不同。这些结果表明，复杂的调节凝集素基因，可能是由NF-κ B相关的转录因子。
Sarcophaga lectin is an immune defense protein which is transcriptionally induced upon immune challenge in the flesh fly, Sarcophaga peregrina. So far, we have revealed that the Sarcophaga lectin gene has multiple NF-kappaB -binding motifs in its promoter. Here we showed that the nuclear extracts from Sarcophaga-derived culture cells, NIH-Sape-4, and larval fat bodies have binding activity to the multiple kappaB motifs in the lectin gene promoter, some of which were responsive to immune stimuli. We also compared the expression profiles of the lectin gene with those of the antibacterial peptide genes from the point of view of inducers, expression tissues and local induction in digestive tracts. In each case, the lectin gene was activated in different manners from other inducible defense genes. These results indicate the complex regulation of the lectin gene, possibly by NF-kappaB -related transcription factors.