IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry.
IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry.
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IR-MALDI-LDI 与离子淌度正交飞行时间质谱联用。
DOI：
10.1021/pr060055l
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发表时间：
2006
影响因子：
4.4
通讯作者：
Schultz,JAlbert
中科院分区：
文献类型：
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作者：
Woods,AminaS;Ugarov,Michael;Jackson,ShelleyN;Egan,Thomas;Wang,Hay-YanJ;Murray,KermitK;Schultz,JAlbert
大多数MALDI仪器使用紫外线激光。我们设计了一台MALDI−IM−OTOF−MS，它既使用了激光泵浦的光学参量振荡器(OPOTEK，λ=2.8−3.2μm，频率为20赫兹)来实现IR−激光或IR−MALDI，又使用了一台用于紫外光的Nd：YLF激光器(晶体激光器，λ=2 49 nm，频率为200 Hz)。离子迁移率(IM)提供了一种从复杂混合物中快速分离和分析生物分子的方法，在这些混合物中，类似化学类型的离子沿着明确定义的“趋势线”落入。我们的数据表明，离子的迁移率允许分解多电荷的单体和多聚体，从而产生纯净的单电荷蛋白质离子光谱，几乎没有化学噪声。此外，我们还证明了在大鼠脑磷脂的直接组织分析中，IR−LDI与IR−MALDI产生了类似的结果。关键词：离子迁移率MALDI·UV激光·IR激光·加合物还原
Most MALDI instrumentation uses UV lasers. We have designed a MALDI−IM−oTOF−MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, λ = 2.8−3.2 μm at 20 Hz) to perform IR−LDI or IR−MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined “trend lines”. Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR−LDI produced similar results as IR−MALDI for the direct tissue analysis of phospholipids from rat brain.Keywords: ion-mobility MALDI • UV lasers • IR lasers • adducts reduction