Bioinformatics and expression analysis of NAC transcription factor genes in Scutellaria baicalensis
Bioinformatics and expression analysis of NAC transcription factor genes in Scutellaria baicalensis
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DOI：
10.4103/wjtcm.wjtcm_7_18
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发表时间：
2018-04
影响因子：
4.4
通讯作者：
Juan Liu;Tiying Chen;Yuan Yuan-Yuan;Junhui Zhou;Yuyang Zhao;Lu‐qi Huang
中科院分区：
文献类型：
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作者：
Juan Liu;Tiying Chen;Yuan Yuan-Yuan;Junhui Zhou;Yuyang Zhao;Lu‐qi Huang
背景资料：NAC作为植物特有的一种转录因子，在植物生长发育、激素水平调节以及对各种逆境胁迫的响应等方面发挥着重要的生物学功能。然而，目前对NAC基因在中草药中的研究还很缺乏。目的：本研究旨在通过生物信息学和表达分析评价黄芩NAC基因的潜在功能，为黄芩黄酮类化合物生物合成的分子调控机制提供依据。黄芩方法：对S.从cDNA文库中获得了黄芩苷的cDNA片段，并利用生物信息学方法对其功能进行了初步研究。从S.用BLAST比较软件对黄芩进行同源性分析。利用开放阅读框架（ORF）finder在线工具预测NAC基因的全长ORF，并利用生物信息学方法分析NAC基因的蛋白质特征。用定量聚合酶链反应检测NAC基因在S.赤霉素GA_3处理的黄芩叶片和不同部位的黄芩叶片。结果：克隆了6个NAC转录因子基因，其中2个具有完整的开放阅读框。本研究克隆的NAC基因主要在甜菊花中表达。黄芩100 μM GA 3处理后，NAC 2、NAC 3、NAC 4、NAC 5、NAC 6的表达量先升高后逐渐降低。同时，S.黄芩表现出较强的相关性。结论：本研究所克隆的NAC主要参与了芍药花中黄酮类化合物的合成。baicalensis; S.黄芩可能参与了PAL 2基因的转录调控，并影响了黄芩根中黄酮类化合物的积累。黄芩本研究结果为进一步了解S.黄芩
Background: NAC, as a unique transcription factor to plants, plays important roles in multiple biological functions, such as regulation of plant growth and development, hormone levels, and responses to various kinds of stresses. However, there is a lack of research of NAC genes in Chinese herbs. Objective: The study aimed to evaluate the potential functions of NAC genes in Scutellaria baicalensis by bioinformatics and expression analysis, and provide evidence of the molecular regulation mechanism involved in flavonoid biosynthesis in S. baicalensis. Methods: The genes of NAC transcription factors in S. baicalensis were obtained from cDNA library and their functions were explored using bioinformatic methods. The NAC genes were screened from the cDNA library of S. baicalensis using BLAST comparison software. Then, the open reading frame (ORF) finder online tool was used to predict the full-length ORFs of NAC genes and their protein characteristics were explored by bioinformatic methods. The expression of NAC genes was then detected by quantitative polymerase chain reaction in different parts of S. baicalensis and different leaves treated by gibberellin GA3 treatment. Results: Six genes of NAC transcription factors were cloned, two of which had complete ORFs. NAC genes cloned in this study were mainly expressed in the flowers of S. baicalensis. The expression levels of NAC2, NAC3, NAC4, NAC5, NAC6 were increased firstly and then decreased gradually after 100 μM GA3 treatment. Meanwhile, some NACs and PAL2 in S. baicalensis showed strong correlation. Conclusion: This study suggested that NACs cloned in this study were mainly regulated the flavonoid biosynthesis in the flowers of S. baicalensis; NAC6 in S. baicalensis might be involved in the regulation of PAL2 transcription and affected the accumulation of flavonoids in the root of S. baicalensis. Our results provided a basis for further understanding the molecular regulation mechanism of flavonoid biosynthesis in S. baicalensis.