Spectroscopic analysis of immobilised redox enzymes under direct electrochemical control
Spectroscopic analysis of immobilised redox enzymes under direct electrochemical control
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DOI：
10.1039/c1cc15871f
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Vincent, Kylie A.
中科院分区：
文献类型：
--
作者：
Ash, Philip A.;Vincent, Kylie A.
本文综述了在直接、非介导电化学控制下电极固定化酶光谱分析的最新进展。这些方法结合了一套光谱方法，可用于表征蛋白质中的结构，电子和配位变化，并对复杂的氧化还原酶进行精确控制，这可以在蛋白质膜电化学中实现，其中固定的蛋白质分子直接与电极交换电子。这种组合在高活性酶的研究中特别强大，其中氧化还原状态甚至可以在快速电催化周转下进行控制。我们研究的例子中，紫外可见光，红外，拉曼和MCD光谱已结合直接电化学探测氧化还原依赖的化学，并考虑未来的机会“直接”光谱电化学固定化酶。
This article reviews recent developments in spectroscopic analysis of electrode-immobilised enzymes under direct, unmediated electrochemical control. These methods unite the suite of spectroscopic methods available for characterisation of structural, electronic and coordination changes in proteins with the exquisite control over complex redox enzymes that can be achieved in protein film electrochemistry in which immobilised protein molecules exchange electrons directly with an electrode. This combination is particularly powerful in studies of highly active enzymes where redox states can be controlled even under fast electrocatalytic turnover. We examine examples in which UV-visible, IR, Raman and MCD spectroscopy have been combined with direct electrochemistry to probe redox-dependent chemistry, and consider future opportunities for 'direct' spectroelectrochemistry of immobilised enzymes.