The consistent use of organic solvents for purification of phospholipids from brain tissue effectively removes scrapie infectivity.
The consistent use of organic solvents for purification of phospholipids from brain tissue effectively removes scrapie infectivity.
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持续使用有机溶剂从脑组织中纯化磷脂可有效消除瘙痒病的感染性。
DOI：
10.1006/biol.1994.1032
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
C. Gibbs
中科院分区：
文献类型：
--
作者：
A. Di Martino;J. Safar;C. Gibbs
评估了从脑组织中纯化磷脂的程序在消除瘙痒病病原体感染方面的功效。该工艺的主要特点是仅使用有机溶剂来提取和纯化磷脂。使用 263 K 感染的仓鼠大脑作为源材料，通过体内动物生物测定对原始程序的缩小版进行实验。最终制剂中不存在任何可检测到的感染性，这表明有机溶剂萃取对于从瘙痒病样物质中纯化和净化生物制品是可接受的，前提是仅使用有机相。
A procedure for the purification of phospholipids from brain tissue was evaluated for its efficacy in eliminating scrapie agent infectivity. The key feature of the process was that phospholipids were extracted and purified by exclusive use of organic solvents. Experiments were done by in vivo animal bioassay on a scaled-down version of the original procedure using 263 K-infected hamster brains as source material. The absence of any detectable infectivity in the final preparations indicated that organic solvent extraction is acceptable for purification and decontamination of biological products from scrapie-like agents provided that only the organic phase is used.