Developmental Change in the Glycosaminoglycan Composition of the Rat Brain
Developmental Change in the Glycosaminoglycan Composition of the Rat Brain
复制标题
大鼠大脑糖胺聚糖组成的发育变化
DOI：
10.1111/j.1471-4159.1986.tb04540.x
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发表时间：
1986
影响因子：
4.7
通讯作者：
R. Shoji
中科院分区：
文献类型：
--
作者：
A. Oohira;F. Matsui;M. Matsuda;R. Shoji
摘要：糖胺多聚糖(GAG)是从出生前和出生后的大鼠脑中分离得到的。大脑中的GAG含量，基于DNA的数量，在怀孕第13天到第15天期间保持不变。15d后，GAG含量开始增加，10d时达到高峰。胎鼠脑内透明质酸(HA)是主要的GAG(占总数的60%)，出生后相对含量下降。相反，硫酸软骨素的相对含量随着发育而增加，在出生后20天时达到成体水平。硫酸乙酰肝素(HS)是发育早期胎鼠脑中主要的硫酸盐化GAG，但约占出生后脑中GAG总量的10%。除了这些GAG，还从快速生长的大鼠脑中分离出了一种聚唾液糖基糖偶联物。这三个GAG可以从新生大鼠的小脑、大脑或脑干中分离出来。在大脑的每个不同区域，都观察到了与年龄密切相关的GAG成分的变化。这种发育变化可能与大脑的形态发生或成熟有关。
Abstract: Glycosaminoglycans (GAGs) were isolated from the brains of pre‐ and postnatal rats. The GAG content of the brain, based on the amount of DNA, was constant during the period from day 13 to day 15 of gestation. After day 15, the GAG content began to increase and reached a plateau by 10 days after birth. Hyaluronate (HA) was the main GAG (> 60% of the total) in the fetal rat brain, and the relative amount of HA decreased after birth. Conversely, the relative amount of chondroitin sulfate increased with development and reached the adult level by 20 days after birth. Heparan sulfate (HS) was the major sulfated GAG in the fetal rat brain at early developmental stages, but HS accounted for approximately 10% of the total GAG in the postnatal brains. In addition to these GAGs, a polysialosyl glycoconjugate was isolated from rapidly growing brains of the rat. These three GAGs could be isolated either from the cerebellum, cerebrum, or brainstem of the newborn rat. A closely similar age‐related change in the GAG composition was observed in each of these different regions of the brain. The developmental change could be implicated in morphogenesis or maturation of the brain.
脑硫酸乙酰肝素蛋白多糖的分离和表征。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Klinger,MM;Margolis,RU;Margolis,RK
通讯作者：
Margolis,RK
硫酸软骨素蛋白多糖和脑可溶性糖蛋白的分级和特性。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kiang,WL;Margolis,RU;Margolis,RK
通讯作者：
Margolis,RK