Overexpression and Suppression of Violaxanthin De-epoxidase Affects the Sensitivity of Photosystem II Photoinhibition to High Light and Chilling Stress in Transgenic Tobacco
Overexpression and Suppression of Violaxanthin De-epoxidase Affects the Sensitivity of Photosystem II Photoinhibition to High Light and Chilling Stress in Transgenic Tobacco
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紫黄素脱环氧化酶的过表达和抑制影响转基因烟草中光系统 II 光抑制对强光和低温胁迫的敏感性
DOI：
10.1111/j.1744-7909.2010.00891.x
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发表时间：
2010-03-01
影响因子：
11.4
通讯作者：
Meng, Qing-Wei
中科院分区：
文献类型：
--
作者：
Gao, Shan;Han, Han;Meng, Qing-Wei
在花菜花叶病毒35S启动子（35S- camv）的控制下，用番茄（Lycopersicon esculentum）紫黄质去环氧酶基因（LeVDE）的正反义构建体转化烟草（Nicotiana tabacum）。逆转录聚合酶链反应和western blot分析表明，外源基因已被整合到烟草基因组中。采用野生型（WT）、转义系T-1-24（+）和反义系T-1-17（-）进行生理测定。在强光和低辐照冷胁迫下，WT的(A+Z)/(V+A+Z)和非光化学猝灭比低于感官植物，高于反义植物。光合系统II的最大光化学效率（PSII） （Fv/Fm）和净光合速率（Pn）在所有品系中下降幅度较小，反义品系的Fv/Fm和Pn下降最为明显。这些结果表明，转基因植物中紫黄质去环氧酶基因的表达影响了PSII光抑制对强光和低温胁迫的敏感性。
Tobacco (Nicotiana tabacum) transformed with the sense and antisense constructs of tomato (Lycopersicon esculentum) violaxanthin de-epoxidase gene (LeVDE) was obtained under the control of the cauliflower mosaic virus 35S promoter (35S-CaMV). Reverse transcription-polymerase chain reaction and western blot analysis demonstrated that the exogenous gene was integrated into the tobacco genome. Wild type (WT), the sense-transgenic line T-1-24(+) and the antisense-transgenic line T-1-17(-) were used for physiological measurement. The ratio of (A+Z)/(V+A+Z) and non-photochemical quenching in WT were lower than that in sense plants and higher than that in antisense ones under high light and chilling stress with low irradiance. The maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm) and the net photosynthetic rate (Pn) in the sense line decreased less, while Fv/Fm and Pn in the antisense line decreased most obviously among all lines. These results suggest that the expression of the violaxanthin de-epoxidase gene in transgenic plants affects the sensitivity of PSII photoinhibition to high light and chilling stress.