Biodistribution and dosimetry of [18F]fluorodeoxyglucose labelled leukocytes in normal human subjects
Biodistribution and dosimetry of [18F]fluorodeoxyglucose labelled leukocytes in normal human subjects
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DOI：
10.1097/00006231-200208000-00004
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发表时间：
2002-08-01
影响因子：
1.5
通讯作者：
Thorson, LM
中科院分区：
文献类型：
--
作者：
Forstrom, LA;Dunn, WL;Thorson, LM
进行本研究的目的是评估正常人类受试者的[F-18]氟脱氧葡萄糖白细胞 (F-18-FDG WBC) 剂量测定。使用先前报道的方法，从四名正常志愿者制备自体白细胞的混合细胞悬液。通过与 F-18-FDG 在 37°C 下孵育 20 分钟，在肝素盐水中标记白细胞。洗涤并重悬后，通过静脉注射施用F-18-FDG WBC (225-315 MBq)。使用具有 511 keV 准直的 GE Varicam 在 0.5、1、2,4 和 6 小时进行全身成像。在相应时间获取血样以及分次尿液收集。全身前部和后部图像用于计算器官剂量测定。 F-18-FDG WBC 的摄取主要发生在网状内皮系统内。血浆活性、尿排泄（6 小时时为 9.9+/-2.3%）和脑摄取（1.7+/-0.4%）与 F-18-FDG 的部分洗脱一致。注射后 5-6 小时进行的正电子发射断层扫描成像产生了网状内皮摄取的高质量图像。 225-250 MBq 剂量的 F-18-FDG WBC 的全身和器官剂量测定与 In-111 oxine 标记白细胞常规剂量的报告结果相当。 18F-FDG WBC 作为炎症性疾病正电子发射断层扫描成像剂的进一步研究似乎有必要。 （（C）2002 年利平科特·威廉姆斯·威尔金斯）。
This study was performed in order to assess [F-18]fluorodeoxyglucose white blood cell (F-18-FDG WBC) dosimetry in normal human subjects. Using previously reported methods, mixed cell suspensions of autologous leukocytes were prepared from four normal volunteers. Leukocytes were labelled in heparin-saline by incubation with F-18-FDG at 37degreesC for 20 min. After washing and resuspension, F-18-FDG WBCs (225-315 MBq) were administered by intravenous injection. Whole-body imaging was performed at 0.5, 1, 2,4 and 6 h using a GE Varicam with 511 keV collimation. Blood samples were obtained at corresponding times as well as fractionated urinary collection. Whole-body anterior and posterior images were used for calculation of organ dosimetry. Uptake of F-18-FDG WBCs occurred predominantly within the reticuloendothelial system. Plasma activity, urinary excretion (9.9+/-2.3% at 6 h), and brain uptake (1.7+/-0.4%) were consistent with partial elution of F-18-FDG. Positron emission tomography imaging performed at 5-6 h after injection yielded good quality images of reticulo-endothelial uptake. Whole-body and organ dosimetry for F-18-FDG WBCs in doses of 225-250 MBq are comparable with reported results for conventional doses of In-111 oxine labelled leukocytes. Further studies of 18F-FDG WBC as an agent for positron emission tomography imaging of inflammatory disease appear warranted. ((C) 2002 Lippincott Williams Wilkins).