HU NEURONAL PROTEINS ARE EXPRESSED IN PROLIFERATING NEUROGENIC CELLS
HU NEURONAL PROTEINS ARE EXPRESSED IN PROLIFERATING NEUROGENIC CELLS
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DOI：
10.1002/neu.480250206
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发表时间：
1994-02-01
期刊：
影响因子：
--
通讯作者：
WESTON, JA
中科院分区：
文献类型：
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作者：
MARUSICH, MF;FURNEAUX, HM;WESTON, JA
我们利用免疫化学技术来研究 Hu 蛋白的发育表达，Hu 蛋白是脊椎动物中 RNA 结合蛋白的神经元特异性家族。先前的研究表明这些蛋白质可能在神经元发育和维持中发挥重要作用。在本研究中，我们开发了一种单克隆抗体 (MAb 16A11)，它与所有已知 Hu 基因（包括 HuD、HuC 和 Hel-N1）的基因产物中存在的表位特异性结合。使用 DNA 前体类似物溴脱氧尿苷 (BrdU) 与 MAb 16All 的短暂脉冲（1-2 小时），我们在体内新生感觉神经节和交感神经节以及培养的神经嵴细胞群中观察到了 Hu(+)/BrdU(+) 细胞。此外，神经管中少数Hu(+)细胞的BrdU(+)不明确。我们得出的结论是，Hu(+)细胞首先出现在鸟类神经原性群体中，即周围神经系统神经元生日之前，以及中枢神经系统退出有丝分裂周期时。与这些结论一致，我们还观察到神经嵴衍生细胞均为Hu(+)且处于细胞周期中期。我们认为 Hu 蛋白在神经源性分化的早期发挥作用。 (C) 1994 约翰威利父子公司
We have utilized immunochemical techniques to investigate the developmental expression of the Hu proteins, a neuron-specific family of RNA binding proteins in vertebrates. Previous work suggests that these proteins may play an important role in neuronal development and maintenance. For the present study, we developed a monoclonal antibody (MAb 16A11) that binds specifically to an epitope present in gene products of all known Hu genes, including HuD, HuC, and Hel-N1. Using brief pulses (1-2 h) of the DNA precursor analog bromodeoxyuridine (BrdU) in conjunction with MAb 16All, eve observed Hu(+)/BrdU(+) cells in nascent sensory and sympathetic ganglia in vivo, and in populations of cultured neural crest cells. In addition, a few Hu(+) cells were ambiguously BrdU(+) in the neural tube. We conclude that Hu(+) cells first appear in avian neurogenic populations immediately before neuronal birthdays in the peripheral nervous system, and at the time of withdrawal from the mitotic cycle in the central nervous system. Consistent with these conclusions, we have also observed neural crest-derived cells that are both Hu(+) and in metaphase of the cell cycle. We suggest that Hu proteins function early in neurogenic differentiation. (C) 1994 John Wiley and Sons, Inc.