Regulation of release of antibacterials from stressed scleractinian corals
Regulation of release of antibacterials from stressed scleractinian corals
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DOI：
10.1111/j.1574-6968.2009.01590.x
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发表时间：
2009-06-01
影响因子：
2.1
通讯作者：
Rosenberg, Eugene
中科院分区：
文献类型：
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作者：
Geffen, Yuval;Ron, Eliora Z.;Rosenberg, Eugene
最近，我们发现，机械应力油石珊瑚引起抗菌物质的快速释放（称为珊瑚抗菌活性，或CAA），这杀死了各种细菌物种，包括珊瑚病原体溶珊瑚弧菌。我们现在报告的研究石油CAA释放的监管压力石珊瑚。珊瑚会反复释放高度活跃的CAA作为连续压力诱导的结果。将珊瑚碎片从一个烧杯转移到另一个烧杯中19次，每次10 min后进行应力诱导。在前4到5次转移期间，释放的抗菌活性水平下降。在第五次转移后，珊瑚在剩余的实验中继续释放CAA，没有显著减少。很明显，CAA的释放受到反馈抑制的下调，这取决于周围水体中CAA的浓度。通过将CAA处理的V coralliilyticus与周围的水分离，结果表明CAA以化学计量的方式不可逆地与细菌细胞结合。大约4 × 10（2）个细菌细胞足以结合CAA的IU。珊瑚细菌对CAA的抗性高于海水细菌，说明CAA具有一定的生态学作用。CAA的释放是从珊瑚的粘液层去除后，和粘液本身含有抗菌活性。
Recently, we showed that mechanical stress oil scleractinian (stony) corals caused a rapid release of antibacterial material (referred to as coral antibacterial activity, or CAA), which killed various bacterial species, including the coral pathogen Vibrio coralliilyticus. We now report on studies oil the regulation of CAA release from stressed scleractinian corals. Corals call repeatedly release highly active CAA as a result Of sequential stress inductions. Coral fragments were transferred 19 times from one beaker into another with a stress induction each time after 10 min. There was a decrease ill the level of antibacterial activity released during the first four to five transfers. After the fifth transfer, the corals kept releasing CAA for the rest of the experiment with no significant decrease. Apparently, the release of CAA is downregulated by feedback inhibition, depending oil the concentration of CAA in the surrounding water. By separating CAA-treated V coralliilyticus from the surrounding water, it was shown that CAA was bound irreversibly to bacterial cells in a stoichiometric manner. Approximately 4 x 10(2) bacterial cells were sufficient to bind I U of CAA. Resident coral bacteria were more resistant to CAA than bacteria isolated from seawater, Suggesting all ecological role for CAA. CAA release was obtained from corals after removal of the mucus layer, and the mucus itself contained antibacterial activity.