Enhancement of Secondary Cell Wall Formation in Poplar Xylem Using a Self-Reinforced System of Secondary Cell Wall-Related Transcription Factors.
Enhancement of Secondary Cell Wall Formation in Poplar Xylem Using a Self-Reinforced System of Secondary Cell Wall-Related Transcription Factors.
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DOI：
10.3389/fpls.2022.819360
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发表时间：
2022
影响因子：
5.6
通讯作者：
Demura T
中科院分区：
文献类型：
--
作者：
Nakano Y;Endo H;Gerber L;Hori C;Ihara A;Sekimoto M;Matsumoto T;Kikuchi J;Ohtani M;Demura T
木质部次生细胞壁是木本植物最大的碳汇器官之一，被认为是一种很有前途的生物燃料和生物材料的可持续生物资源。为促进杨树(Pillius sp.)木质部，我们从拟南芥中开发了一个与SCW相关的转录因子的自我增强系统，包括维管相关的NAC-DOMAIN7(VND7)、次生壁相关的NAC结构域蛋白1/NAC次生壁增厚促进因子3(SND1/NST3)和MYB46。在该系统中，这些转录因子与三果杨CesA18(PtCesA18)基因启动子控制的反式激活域VP16融合并表达，形成了嵌合基因PtCesA18pro：：AtVND7：VP16、PtCesA18pro：：AtSND1：VP16和PtCesA18pro：：AtMYB46：VP16。PtCesA18启动子在产生SCW的组织中是活跃的，可以被AtVND7、AtSND1和AtMYB46调控，因此，PtCesA18pro：：AtVND7：VP16、PtCesA18pro：：AtSND1：VP16和PtCesA18pro：：AtMYB46：VP16的表达水平有望在产生SCW的组织中上调。转PtCesA18pro：：AtSND_1：VP16或PtCesA18Pro：：AtMYB46：VP16的转基因杂交杨树(Popus tremula × tremuloids T89)在无菌半强度Murashige和Skoog培养基中生长时，次生木质部细胞的细胞壁增厚显著增加，而PtCesA18Pro：：AtVND7：VP16植株的木质部形成受阻，这可能是由于木质部细胞程序性死亡增加所致。经过驯化后，将转基因植株从无菌生长介质转移到温室土壤中，只有PtCesA18pro：：AtMYB46：VP16杨树存活下来。核磁共振足迹细胞壁分析和酶糖化分析表明，PtCesA18Pro：：AtMYB46：VP16影响细胞壁性质，如木素的丁香基(S)和愈创木基(G)单位的比率，木质素β芳基醚和树脂醇键的丰度，以及半纤维素的乙酰化水平。综上所述，这些数据表明，我们已经创建了一个使用SCW相关转录因子来促进SCW积累的自我增强系统。
The secondary cell wall (SCW) in the xylem is one of the largest sink organs of carbon in woody plants, and is considered a promising sustainable bioresource for biofuels and biomaterials. To enhance SCW formation in poplar (Populus sp.) xylem, we developed a self-reinforced system of SCW-related transcription factors from Arabidopsis thaliana, involving VASCULAR-RELATED NAC-DOMAIN7 (VND7), SECONDARY WALL-ASSOCIATED NAC-DOMAIN PROTEIN 1/NAC SECONDARY WALL THICKENING-PROMOTING FACTOR3 (SND1/NST3), and MYB46. In this system, these transcription factors were fused with the transactivation domain VP16 and expressed under the control of the Populus trichocarpa CesA18 (PtCesA18) gene promoter, creating the chimeric genes PtCesA18pro::AtVND7:VP16, PtCesA18pro::AtSND1:VP16, and PtCesA18pro::AtMYB46:VP16. The PtCesA18 promoter is active in tissues generating SCWs, and can be regulated by AtVND7, AtSND1, and AtMYB46; thus, the expression levels of PtCesA18pro::AtVND7:VP16, PtCesA18pro::AtSND1:VP16, and PtCesA18pro::AtMYB46:VP16 are expected to be boosted in SCW-generating tissues. In the transgenic hybrid aspens (Populus tremula × tremuloides T89) expressing PtCesA18pro::AtSND1:VP16 or PtCesA18pro::AtMYB46:VP16 grown in sterile half-strength Murashige and Skoog growth medium, SCW thickening was significantly enhanced in the secondary xylem cells, while the PtCesA18pro::AtVND7:VP16 plants showed stunted xylem formation, possibly because of the enhanced programmed cell death (PCD) in the xylem regions. After acclimation, the transgenic plants were transferred from the sterile growth medium to pots of soil in the greenhouse, where only the PtCesA18pro::AtMYB46:VP16 aspens survived. A nuclear magnetic resonance footprinting cell wall analysis and enzymatic saccharification analysis demonstrated that PtCesA18pro::AtMYB46:VP16 influences cell wall properties such as the ratio of syringyl (S) and guaiacyl (G) units of lignin, the abundance of the lignin β-aryl ether and resinol bonds, and hemicellulose acetylation levels. Together, these data indicate that we have created a self-reinforced system using SCW-related transcription factors to enhance SCW accumulation.
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影响因子：
10.5
作者：
Kubo, M;Udagawa, M;Demura, T
通讯作者：
Demura, T
影响因子：
11.6
作者：
Mitsuda, N;Seki, M;Ohme-Takagi, M
通讯作者：
Ohme-Takagi, M
影响因子：
4
作者：
Akiyoshi, Nobuhiro;Nakano, Yoshimi;Demura, Taku
通讯作者：
Demura, Taku
影响因子：
3
作者：
Davison, Brian H.;Drescher, Sadie R.;Nghiem, Nhuan P.
通讯作者：
Nghiem, Nhuan P.
影响因子：
1.2
作者：
Mitra, Prajakta Pradhan;Loque, Dominique
通讯作者：
Loque, Dominique