The Drosophila homolog of C-elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole
The Drosophila homolog of C-elegans PAR-1 organizes the oocyte cytoskeleton and directs oskar mRNA localization to the posterior pole
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DOI：
10.1016/s0092-8674(00)80848-x
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发表时间：
2000-05-12
期刊：
影响因子：
64.5
通讯作者：
St Johnston, D
中科院分区：
文献类型：
--
作者：
Shulman, JM;Benton, R;St Johnston, D
In C.在线虫中，PAR-1激酶定位于受精卵的后部，并且是前后轴形成所需的。在这里，我们报告说，果蝇PAR-1同源物定位到后面的卵母细胞与奥斯卡mRNA。此外，par-1突变体表现出一种新的极性表型，其中bicoid mRNA通常在前部积累，但oskar mRNA被重定向到卵母细胞的中心，导致胚胎图案缺陷。这些表型来自卵母细胞微管细胞骨架的解体，与哺乳动物PAR-1同源物调节微管动力学的报道一致。因此，果蝇PAR-1可能重塑卵母细胞微管网络，以定义为奥斯卡本地化的网站后部。这些结果表明果蝇和C.优美的
In C. elegans, the PAR-1 kinase is localized to the posterior of the zygote and is required for anterior-posterior axis formation. Here, we report that a Drosophila PAR-1 homolog localizes to the posterior of the oocyte with oskar mRNA. Furthermore, par-1 mutants show a novel polarity phenotype in which bicoid mRNA accumulates normally at the anterior, but oskar mRNA is redirected to the center of the oocyte, resulting in embryonic patterning defects. These phenotypes arise from a disorganization of the oocyte microtubule cytoskeleton, consistent with reports that mammalian PAR-1 homologs regulate microtubule dynamics. Thus, Drosophila PAR-1 may remodel the oocyte microtubule network to define the posterior as the site for oskar localization. These results identify a molecular parallel between anterior-posterior polarization in Drosophila and C. elegans.