Protective effect of melatonin against head trauma-induced hippocampal damage and spatial memory deficits in immature rats
Protective effect of melatonin against head trauma-induced hippocampal damage and spatial memory deficits in immature rats
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DOI：
10.1016/j.neulet.2005.05.055
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发表时间：
2005-09-16
影响因子：
2.5
通讯作者：
Ozkan, H
中科院分区：
文献类型：
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作者：
Ozdemir, D;Tugyan, K;Ozkan, H
众所周知，脑外伤可导致海马损伤导致认知功能障碍。在本研究中，我们的目的是证明褪黑激素对海马损伤和空间记忆障碍的影响在7日龄大鼠受到挫伤。在诱导创伤性损伤后立即以5或20 mg/kg体重的剂量腹腔内注射褪黑激素。用甲酚紫染色和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法检测海马神经元损伤。在Morris水迷宫中评估空间记忆性能。褪黑激素显著减弱海马CA 1、CA 3区和齿状回中创伤诱导的神经元死亡，并改善空间记忆缺陷，这在5-20 mg/kg剂量下同样有效。目前的研究结果表明，褪黑激素是一个非常有前途的代理，以防止不利的结果，创伤性脑损伤的幼儿。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
It is well known that head trauma induces the cognitive dysfunction resulted from hippocampal damage. In the present study, we aimed to demonstrate the effect of melatonin on hippocampal damage and spatial memory deficits in 7-day-old rat pups subjected to contusion injury. Melatonin was injected intraperitoneally at the doses of 5 or 20 mg/kg of body weight immediately after induction of traumatic injury. Hippocampal damage was examined by cresyl violet staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Spatial memory performance was assessed in the Morris water maze. Melatonin significantly attenuated trauma-induced neuronal death in hippocampal CA1, CA3 regions and dentate gyrus, and improved spatial memory deficits, which was equally effective at doses of 5-20 mg/kg. The present results suggest that melatonin is a highly promising agent for preventing the unfavorable outcomes of traumatic brain injury in young children. (c) 2005 Elsevier Ireland Ltd. All rights reserved.