Engineering Biomolecular Switches for Dynamic Metabolic Control.
Engineering Biomolecular Switches for Dynamic Metabolic Control.
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DOI：
10.1007/10_2016_9
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Chengwei Ma;Li-Bang Zhou;A. Zeng
中科院分区：
文献类型：
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作者：
Chengwei Ma;Li-Bang Zhou;A. Zeng
生物有机体已被开发为多种化合物的生产宿主。为了提高生物生产的效率，生物体内的代谢途径通常被各种基因修饰所操纵。然而，在将底物转化为所需产品的过程中，瓶颈可能是由不同水平的细胞调控引起的。因此，根据培养过程的需要动态调节代谢途径是开发有效生物工艺的必要条件，也是代谢工程和合成生物学的主要挑战。为此，能够感知具有不同响应类型和动态范围的代谢物细胞内浓度的可切换生物分子引起了人们的极大兴趣。本章综述了生物分子开关的最新进展及其通过动态控制代谢通量来改善生物生产的应用。对生物开关的进一步研究及其在工业菌株开发中的应用进行了讨论。
Living organisms have been exploited as production hosts for a large variety of compounds. To improve the efficiency of bioproduction, metabolic pathways in an organism are usually manipulated by various genetic modifications. However, bottlenecks during the conversion of substrate to a desired product may result from cellular regulations at different levels. Dynamic regulation of metabolic pathways according to the need of cultivation process is therefore essential for developing effective bioprocesses, but represents a major challenge in metabolic engineering and synthetic biology. To this end, switchable biomolecules which can sense the intracellular concentrations of metabolites with different response types and dynamic ranges are of great interest. This chapter summarizes recent progress in the development of biomolecular switches and their applications for improvement of bioproduction via dynamic control of metabolic fluxes. Further studies of bioswitches and their applications in industrial strain development are also discussed.