Isolation and characterization of Ni-specific T cell clones from patients with Ni-contact dermatitis.
Isolation and characterization of Ni-specific T cell clones from patients with Ni-contact dermatitis.
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镍接触性皮炎患者镍特异性 T 细胞克隆的分离和表征。
DOI：
10.4049/jimmunol.135.6.3929
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发表时间：
1985
影响因子：
4.4
通讯作者：
A. Lanzavecchia
中科院分区：
文献类型：
--
作者：
F. Sinigaglia;D. Scheidegger;G. Garotta;R. Scheper;M. Pletscher;A. Lanzavecchia
从两名镍接触性皮炎患者中分离出镍特异性 T 淋巴细胞克隆 (TLC)。所有分离的 TLC 都需要组织相容性抗原呈递细胞 (APC) 和 Ni 来诱导增殖。通过使用一组 HLA 型 Epstein Barr 病毒转化的 B 细胞（EBV-B 细胞）作为 APC 和单克隆抗 DR 抗体，这些克隆被证明能够在 HLA II 类决定簇的背景下识别 Ni。所有分离的克隆都是 OKT3+、OKT4+、OKT8-。在 Ni 存在的情况下，它们多克隆地激活自体 B 细胞，在 Ni 和自体 EBV-B 细胞存在的情况下，它们产生 IL 2 和非常高水平的 IFN-γ。 Ni特异性克隆应该有助于识别T细胞识别的Ni诱导抗原。
Ni-specific T lymphocyte clones (TLC) were isolated from two patients with Ni-contact dermatitis. All of the isolated TLC required both histocompatible antigen-presenting cells (APC) and Ni for induction of proliferation. By using a panel of HLA-typed Epstein Barr virus-transformed B cells (EBV-B cells) as APC and monoclonal anti-DR antibody, the clones were shown to recognize Ni in the context of HLA class II determinants. All of the clones that were isolated are OKT3+, OKT4+, OKT8-. In the presence of Ni, they polyclonally activate autologous B cells, and in the presence of Ni and autologous EBV-B cells, they produce IL 2 and very high levels of IFN-gamma. The Ni-specific clones should be helpful in the identification of the Ni-induced antigen which is recognized by T cells.