ISOLATION AND SEROTYPING OF STREPTOCOCCUS-MUTANS FROM TEETH AND FECES OF CHILDREN
ISOLATION AND SEROTYPING OF STREPTOCOCCUS-MUTANS FROM TEETH AND FECES OF CHILDREN
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DOI：
10.1128/jcm.11.4.314-318.1980
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发表时间：
1980-01-01
影响因子：
9.4
通讯作者：
KOTANI, S
中科院分区：
文献类型：
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作者：
HAMADA, S;MASUDA, N;KOTANI, S
在4-9岁的29名龋病活动性患者中，有10人的粪便中检出变形链球菌。变形链球菌占兼性厌氧链球菌总数的百分比为0-72.5%。然后，从已知粪便中含有变形链球菌的10名受试者的健康牙齿的牙菌斑和龋齿的牙本质中分离出变形链球菌。用免疫扩散法比较不同血清型的牙科和粪便分离株的频率分布。共分离出1047株细菌，其中粪便分离株290株，牙菌斑分离株289株，龋齿分离株468株，其中c型最为常见(约占66%)。也发现了d、e、f和g型分离株，但出现的频率要低得多。在同一受试者的牙齿和粪便样本中，偶尔会发现多种血清型的变形链球菌。有2名受试者在受检人群中发现了相对罕见的变形链球菌血清型，即e、f或g血清型，主要存在于他们的粪便和牙齿样本中，以及他们的兄弟姐妹和母亲的样本中，这表明变形链球菌是在家庭内传播的。
S. mutans were detected in the feces from 10 of 29 caries-active patients, aged 4-9 yr. The percentage of S. mutans to the total counts of facultatively anaerobic streptococci on mitis salivarius agar (Difco Laboratories) varied from 0-72.5%. S. mutans were then isolated from dental plaque of sound teeth and carious dentin of the 10 subjects known to harbor S. mutans in the feces. The frequency distribution of various serotypes of these dental and fecal isolates of S. mutans was compared by the immunodiffusion technique. Of the total 1047 isolates (290 isolates from feces, 289 from dental plaque and 468 from carious dentin), type c isolates were most prevalent (about 66%). Serotype d, e, f and g isolates were also found but in far lower frequencies. Plural serotypes of S. mutans were occasionally found in dental and fecal samples of a single subject. For 2 subjects, relatively rare serotypes of S. mutans in the population examined, serotype e, f or g, were predominantly found in their fecal and dental samples and those of their siblings and mother, suggesting an intrafamilial transmission of S. mutans.