EFFECTS OF NUTRIENT DEPRIVATION ON VIBRIO-CHOLERAE
EFFECTS OF NUTRIENT DEPRIVATION ON VIBRIO-CHOLERAE
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DOI：
10.1128/aem.46.4.930-940.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
HOOD, MA
中科院分区：
文献类型：
--
作者：
BAKER, RM;SINGLETON, FL;HOOD, MA
Environmental and clinical strains of V. cholerae were exposed to nutrient-free artificial seawater and filtered natural seawater microcosms for selected time intervals and examined for changes in cell morphology and number. Cells observed by transmission EM and epifluorescence microscopy underwent gross alterations in cell morphology with time of exposure. The vibrioid cells decreased in volume by 85% and developed into small coccoid forms surrounded by remnant cell walls. The initial number of cells inoculated into nutrient-free microcosms (culturable count and direct viable count) increased 2.5 log within 3 days, and even after 75 days the number of viable cells was still 1-2 log10 higher than the initial inoculum size. Nutrient-depleted coccoid-shaped cells were restored to normal size and assumed a bacillary shape within 2 h and began to divide within 5 h after nutrient supplementation. The increase in cell number and decrease in cell volume under nutrient-depleted conditions, and the rapid growth response after nutrient supplementation, may describe some of the survival mechanisms of V. cholerae in the aquatic environment.
