Reconstruction of irradiated bone segmental defects with a biomaterial associating MBCP plus ®, microstructured collagen membrane and total bone marrow grafting: An experimental study in rabbits
Reconstruction of irradiated bone segmental defects with a biomaterial associating MBCP plus ®, microstructured collagen membrane and total bone marrow grafting: An experimental study in rabbits
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DOI：
10.1002/jbm.a.32274
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发表时间：
2009-12-15
影响因子：
4.9
通讯作者：
Aguado, Eric
中科院分区：
文献类型：
--
作者：
Jegoux, Franck;Goyenvalle, Eric;Aguado, Eric
目前使用的骨组织工程模型距离任何肿瘤学应用还有很长的一段路要走，因为植入后即刻照射会降低其成骨诱导能力。本研究的目的是重建植入后照射的承重骨的节段性临界大小的缺损区。将6只新西兰大白兔立即植入生物材料结合可吸收胶原膜EZ(R)和微孔大孔双相磷酸钙颗粒(MBCP+(R))。在每天的放射治疗后，在4周内，将全自体骨髓(BM)经皮注射到植入物的中心。所有动物在16周时被处死。成功的骨定植被发现已经桥接了整个长度的缺陷。不同水平种植体的骨生长和残留陶瓷的分布一致，表明BM移植物在缺损区中心起到了骨诱导作用。周围可见骨膜样形成，很可能是胶原膜起作用。该模型成功地用种植后即刻分割放射治疗修复了承重骨的大段缺损区。这对癌症相关骨缺损患者的骨组织工程方法具有重要意义。(C)2009年威利期刊公司生物材料91a：1160-1169,2009
The bone tissue engineering models used today are still a long way from any oncologic application as immediate postimplantation irradiation would decrease their osteoinductive potential. The aim of this study was to reconstruct a segmental critical size defect in a weight-bearing bone irradiated after implantation. Six white New Zealand rabbits were immediately implanted with a biomaterial associating resorbable collagen membrane EZ (R) filled and micro-macroporous biphasic calcium phosphate granules (MBCP+(R)). After a daily schedule of radiation delivery, and within 4 weeks, a total autologous bone marrow (BM) graft was injected percutaneously into the center of the implant. All the animals were sacrificed at 16 weeks. Successful osseous colonization was found to have bridged the entire length of the defects. Identical distribution of bone ingrowth and residual ceramics at the different levels of the implant suggests that the BM graft plays an osteoinductive role in the center of the defect. Periosteum-like formation was observed at the periphery, with the collagen membrane most likely playing a role. This model succeeded in bridging a large segmental defect in weight-bearing bone with immediate postimplantation fractionated radiation delivery. This has significant implications for the bone tissue engineering approach to patients with cancer-related bone defects. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 1160-1169, 2009