Susceptibility differences to Cryptosporidium parvum infection in two strains of gamma interferon knockout mice.
Susceptibility differences to Cryptosporidium parvum infection in two strains of gamma interferon knockout mice.
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DOI：
10.2307/3284643
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发表时间：
1998-10
期刊：
影响因子：
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通讯作者：
J. Mead;X. You
中科院分区：
文献类型：
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作者：
J. Mead;X. You
研究了两种伽玛干扰素基因敲除(GKO)小鼠对隐孢子虫感染易感性的差异。将8~10wk的C57BL/6J-IFG和BALB/c-IFG(GKO)雄性小鼠接种不同剂量的感染性卵囊。C57BL/6J-IFG小鼠发生压倒性感染，感染后9-12天死亡。低接种量(1×10(3))对存活时间无显著影响。接种1×10(5)卵囊/只的C57BL/6J-IFG小鼠在感染后第4天(PI)感染强度显著增加，并在接下来的6-7天继续显著增加。大多数C57BL/6J-IFG小鼠体重下降超过15%，并在第10天死亡。相比之下，BALB/c-IFG小鼠发生了中度感染，并从中恢复。BALB/c-IFG小鼠的平均寄生虫载量比C57BL/6J-IFG小鼠低100倍。即使每只小鼠接种1×10(6)个卵囊，小鼠也能存活到实验结束(39天)。BALB/c-IFG小鼠没有表现出显著的体重减轻(或大便稠度差异)。这两个小鼠品系是研究恢复和不恢复免疫机制差异的极佳模型。
Differences in susceptibility to cryptosporidial infections were investigated between 2 strains of gamma interferon knockout (GKO) mice. Male C57BL/6J-Ifg and BALB/c-Ifg (GKO) mice, ages 8-10 wk, were inoculated with infectious oocysts at various doses. C57BL/6J-Ifg mice developed overwhelming infections and died 9-12 days after infection. Low inoculum doses (1 x 10(3)) did not increase the survival time significantly. The infection intensity in C57BL/6J-Ifg mice inoculated with 1 x 10(5) oocysts/mouse increased markedly on day 4 postinfection (PI) and continued to increase significantly over the next 6-7 days. Most of the C57BL/6J-Ifg mice exceeded 15% weight loss and died by day 10 PI. In contrast, BALB/c-Ifg mice developed moderate infections from which they recovered. The average parasite load in the BALB/c-Ifg mice was 100 times lower than in C57BL/6J-Ifg mice. Mice survived until termination of the experiment (39 days) even when 1 x 10(6) oocysts per mouse were used for inoculation. BALB/c-Ifg mice did not exhibit significant weight loss (or difference in stool consistency). These 2 mouse strains make excellent models for studying differences in recovering and nonrecovering immune mechanisms.