Canine gastroenteritis - viral agents in feces from diarrheic and non-diarrheic dogs
Canine gastroenteritis - viral agents in feces from diarrheic and non-diarrheic dogs
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DOI：
10.1590/s0102-09351999000600004
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发表时间：
1999-12-01
影响因子：
0.4
通讯作者：
Linhares, AC
中科院分区：
文献类型：
--
作者：
Homem, VSF;Mendes, YG;Linhares, AC
对25只腹泻犬和8只非腹泻犬的粪便样本进行了电子显微镜检查，以确定可能与胃肠炎，特别是犬胃肠炎复合体有关的候选病毒病原体，试图将病毒病原学与临床和疫苗接种史联系起来。除EM外，还对71份粪便样本进行了ELISA法检测，以检测轮状病毒或腺病毒抗原。在33份标本中，有25份(75.8%)可用EM观察到病毒颗粒。I型病毒样结构和细小病毒是常见的，分别占病毒阳性制剂的44%和36%。在排泄病毒颗粒的狗中，90.9%的狗出现血性腹泻。
Fecal samples from both diarrheic (n=25) and non-diarrheic (n=8) dogs of both sexes, with varying ages and breeds, were examined by electronic microscopy (EM) in order to determine candidate viral pathogens which would be associated with gastroenteritis, particularly the canine gastroenteritis complex in an attempt to correlate viral etiology and clinical and vaccination history. In addition to EM, 71 stool samples were tested by ELISA to detect either rotavirus or adenovirus antigens. Viral particles could be visualized by EM in 25 (75.8%) of the 33 samples. Type-I virus-like structures and parvoviruses were frequently detected, accounting for 44% and 36% of the virus-positive preparations. Bloody diarrhea was noted in 90.9% of dogs that were excreting viral particles.