Preliminary genetic linkage map of Miscanthus sinensis with RAPD markers
Preliminary genetic linkage map of Miscanthus sinensis with RAPD markers
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DOI：
10.1007/s00122-002-0956-7
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发表时间：
2002-11-01
影响因子：
5.4
通讯作者：
Martín, A
中科院分区：
文献类型：
--
作者：
Atienza, SG;Satovic, Z;Martín, A
采用“后代杂交”作图策略，结合随机扩增多态性DNA（RAPD）技术，首次构建了芒草（Miscanthussinensis）（2n = 2x = 38）的遗传图谱。该图谱基于89个个体的远交群体，所述远交群体由来自先前设计的杂交的两个基因型之间的杂交产生。因此，父母双方都是全同胞。已获得相同比例的双亲标记（双亲杂合）和假测交标记（一个亲本杂合，另一个亲本无效）、单亲标记。在89个F1植株中共检测到383个RAPD标记。在这些标记中，257个标记被定位到28个连锁群中，总的图谱长度约为1，074.5 cM，每个标记的平均密度为4.2 cM。在257个标记中，62个标记来自F1.1（P1），63个标记来自F1.7（P7），132个标记为双亲标记。父母双方对地图的贡献是相等的。该图谱为进一步研究苜蓿重要农艺性状的遗传规律提供了有用的工具。在开花、产量、株高、茎粗、矿质成分等方面对中华绒螯蟹进行了研究。提出了后代交叉映射策略，以提高包括双亲的综合地图的开发效率。
We have used an "offspring cross" mapping strategy in combination with the random amplified polymorphic DNA (RAPD) assay to construct the first genetic map of the species Miscanthus sinensis (2n = 2x = 38). This map is based on an outbred population of 89 individuals resulting from the cross between two genotypes from a previously designed cross. Consequently, both parents are fullsibs. The same proportion of bi-parental markers (heterozygotic in both parents) and pseudo-testcross markers (heterozygotic in one parent and null in the other), mono-parental markers, have been obtained. A total of 383 RAPD markers were analysed within the 89 F1 plants. Out of these markers, 257 were mapped into 28 linkage groups which spanned a total map length of around 1,074.5 cM with an average density of 4.2 cM per marker. Out of 257 mapped markers, 62 were inherited from F1.1 (P1), 63 from F1.7 (P7) and 132 were biparental markers. The contribution to the map was equal from both parents. This map provides a useful tool for genetic analyses of agronomically interesting characters in M. sinensis such as flowering, yield, plant height, stem diameter and mineral constitution. The offspring cross mapping strategy is proposed to obtain a higher efficiency in developing integrated maps including both parents.