Superoxide dismutase with heparin prevents increased lung vascular permeability during air emboli in sheep.
Superoxide dismutase with heparin prevents increased lung vascular permeability during air emboli in sheep.
复制标题
超氧化物歧化酶与肝素可防止绵羊空气栓塞期间肺血管通透性增加。
DOI：
10.1152/jappl.1983.55.4.1284
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Staub,NC
中科院分区：
文献类型：
--
作者：
Flick,MR;Hoeffel,JM;Staub,NC
我们研究了牛超氧化物歧化酶对非麻醉绵羊空气栓塞时肺微血管对液体和蛋白质通透性增加的影响。我们测量了肺动脉和左心房压、心输出量、肺淋巴流量、淋巴和血浆蛋白浓度。在空气栓塞实验中，我们连续向每只羊的右心房注入相同剂量的直径为1mm的气泡，使肺血管阻力增加到基线值的2-4倍。我们成对地做实验，这样每只羊都可以作为自己的对照。我们发现未经治疗的绵羊在栓塞期间肺部富含蛋白质的淋巴液增加，表明微血管对液体和蛋白质的渗透性增加。当我们用肝素（300 U/kg）对绵羊进行预处理，并在空气输注前开始静脉注射牛超氧化物歧化酶（1 mg X kg-1 X h-1）时，我们发现血管压力和肺血管阻力与未处理的绵羊没有差异，但栓塞期间经血管液体和蛋白流量的预期增加明显减少（P < 0.0005）。单独使用肝素并不能显著减弱微血管通透性的增加，但我们发现它大大增强了超氧化物歧化酶在空气栓塞期间保持微血管功能完整性的有效性。我们得出结论，超氧阴离子可能由白细胞产生和释放，是导致空气微栓塞期间绵羊肺部通透性增加的微血管损伤的核心因素。
We studied the effects of bovine superoxide dismutase on the increased lung microvascular permeability to fluid and protein during air emboli in unanesthetized sheep. We measured pulmonary arterial and left atrial pressures, cardiac output, lung lymph flow, and lymph and plasma protein concentrations. In air emboli experiments we continuously infused the same dose of air bubbles 1 mm in diameter into the right atrium of each sheep to increase pulmonary vascular resistance to 2-4 times base-line values. We did experiments in pairs so that each sheep served as its own control. We found an increase in protein-rich lymph flow from the lung during embolization in untreated sheep, indicating an increase in microvascular permeability to both fluid and protein. When we pretreated the sheep with heparin (300 U/kg) and infused them with intravenous bovine superoxide dismutase (1 mg X kg-1 X h-1 beginning before the air infusion), we found that vascular pressures and pulmonary vascular resistance were not different from untreated sheep but that the expected increases in transvascular fluid and protein flow during emboli were significantly decreased (P less than 0.0005). Heparin alone did not significantly attenuate the increased microvascular permeability but we found that it greatly enhanced the effectiveness of superoxide dismutase in preserving microvascular functional integrity during air emboli. We conclude that superoxide anion, probably produced and released by leukocytes, is a central factor in the microvascular injury that results in increased permeability in the lungs of sheep during air microembolization.