PROTEINS OF POSTSYNAPTIC DENSITY
PROTEINS OF POSTSYNAPTIC DENSITY
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DOI：
10.1083/jcb.63.2.456
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发表时间：
1974-01-01
影响因子：
7.8
通讯作者：
COTMAN, CW
中科院分区：
文献类型：
--
作者：
BANKER, G;CHURCHILL, L;COTMAN, CW
本文分析了大鼠脑突触后致密物（PSD）的蛋白质组成。蛋白质占PSD级分中材料的90%。基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳，存在两种主要多肽级分。主要多肽级分的分子量为53，000，占PSD蛋白的约45%，并在凝胶上与突触质膜的主要多肽共迁移。另一条多肽带的分子量为97，000，占PSD蛋白的17%，并且不是其他组分的主要成分。其他六种较高分子量（100，000 - 180，000）的多肽始终以少量（各3-9%）存在。PSD部分含有比突触质膜部分稍大量的极性氨基酸和脯氨酸，但通常没有氨基酸是突出的。PSD显然由主要由53，000分子量的单一多肽或一类多肽形成的结构基质组成。该基质中含有少量其他专门蛋白质。突触后密度（PSD）1是中枢神经系统内大多数突触的特征。在前面的论文（Cotman等人，1974年），我们报告说，PSD可以通过洗涤剂处理含有完整突触连接组件的突触膜组分来分离，
An analysis was made of the protein composition of a fraction of postsynaptic densities (PSDs) prepared from rat brain. Protein makes up 90% of the material in the PSD fraction. Two major polypeptide fractions are present, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The major polypeptide fraction has a molecular weight of 53,000, makes up about 45% of the PSD protein, and comigrates on gels with a major polypeptide of the synaptic plasma membrane. The other polypeptide band has a molecular weight of 97,000, accounts for 17% of the PSD protein, and is not a prominent constituent of other fractions. Six other poly peptides of higher molecular weight (100,000-180,000) are consistently present in small amounts (3-9% each). The PSD fraction contains slightly greater amounts of polar amino acids and proline than the synaptic plasma membrane fraction, but no amino acid is usually prominent. The PSD apparently consists of a structural matrix formed primarily by a single polypeptide or class of polypeptides of 53,000 molecular weight. Small amounts of other specialized proteins are contained within this matrix.The postsynaptic density (PSD) 1 is a characteristic feature of most synapses within the central nervous system. In the preceding paper (Cotman et al., 1974) we reported that PSDs may be isolated by detergent treatment of synaptic membrane fractions that contain intact synaptic junctional com-