Determination of the pore size distribution and porosity of aerobic granules using size-exclusion chromatography.
Determination of the pore size distribution and porosity of aerobic granules using size-exclusion chromatography.
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DOI：
10.1016/j.watres.2006.09.015
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发表时间：
2007
期刊：
影响因子：
12.8
通讯作者：
Yuming Zheng;Hanqing Yu
中科院分区：
文献类型：
--
作者：
Yuming Zheng;Hanqing Yu
以聚乙二醇和蒸馏水为溶质和移动的相，采用体积排阻色谱法对不同粒径好氧颗粒污泥的孔径分布和孔隙率进行了研究。好氧颗粒的孔隙率从68%到93%不等，并且以分子量表示的排除极限显示出显著差异。对于直径为0.2-0.6mm的小颗粒，大于137, 000 Da的分子不能通过微孔，而对于直径为0.6-0.9mm的中等颗粒和直径为0.9-1.5mm的大颗粒，其排阻极限分别为76,000和29, 000 Da。颗粒的胞外聚合物可能堵塞孔隙，并可能是导致孔隙率降低的原因。实验结果表明，颗粒污泥的生物活性与有效孔隙率之间存在一定的相关性，排阻色谱法可用于研究好氧颗粒污泥的孔径分布和孔隙率。
The pore size distribution and porosity of aerobic granules with different diameters were evaluated using size-exclusion chromatography, in which polyethylene glycols and distilled water were, respectively, used as solute and mobile phase. The porosity of the aerobic granules varied from 68% to 93% and the exclusion limit, expressed as molecular mass, showed a significant difference. For the small-size granules with a diameter of 0.2–0.6mm, molecules greater than 137,000Da could not penetrate the pores, while the exclusion limits of the middle-size granules with a diameter of 0.6–0.9mm and large-size ones with a diameter of 0.9–1.5mm were 76,000 and 29,000Da, respectively. The extracellular polymeric substances of the granules might clog the pores and might be responsible for the reduced porosity. A correlation between the bioactivity and available porosity of the aerobic granules was found. The experimental results show that the size-exclusion chromatography was appropriate for elucidating the pore size distribution and porosity of the aerobic granules.