TRANSFORMING GROWTH FACTOR-BETA-1 STIMULATES SYNTHESIS OF PROTEOGLYCAN AGGREGATES IN CALF ARTICULAR-CARTILAGE ORGAN-CULTURES
TRANSFORMING GROWTH FACTOR-BETA-1 STIMULATES SYNTHESIS OF PROTEOGLYCAN AGGREGATES IN CALF ARTICULAR-CARTILAGE ORGAN-CULTURES
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DOI：
10.1016/0003-9861(91)90013-9
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发表时间：
1991-04-01
影响因子：
3.9
通讯作者：
MORALES, TI
中科院分区：
文献类型：
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作者：
MORALES, TI
以前的工作表明，转化生长因子-β1（TGF-β1），单独添加到牛软骨器官培养，刺激[35 S]硫酸盐掺入到大分子材料，但没有调查的保真度刺激系统，以维持软骨型蛋白聚糖的合成。本文提供了软骨细胞在TGF-β1刺激下合成适当蛋白多糖基质的证据：（i）透明质酸和蛋白多糖单体合成协调增加，（ii）组装连接稳定的蛋白多糖聚集体，（iii）合成混合硫酸软骨素/硫酸角质素单体物质，（iv）蛋白质核心合成增加。当将在TGF-β1刺激下合成的蛋白聚糖与在基础条件下合成的蛋白聚糖进行比较时，观察到糖基化模式的一些变化。因此，分别将TGF-β1与基础样品进行比较，单体较大（Kavon Sepharose CL-2B = 0.29 vs 0.41），硫酸软骨素链较长约3.5 kDa，硫酸角质素中总糖胺聚糖的百分比略有增加，从约4%（基础）增加到约6%，未硫酸化的二糖从28%（基础）减少到12%。所有这些变化都是在更阴离子蛋白聚糖的方向上。由于蛋白聚糖赋予软骨基质弹性的能力与其阴离子性质直接相关，因此这些变化可能对组织功能具有有益影响。
Previous work showed that transforming growth factor-β1 (TGF-β1), added alone to bovine cartilage organ cultures, stimulated [35S]sulfate incorporation into macromolecular material but did not investigate the fidelity of the stimulated system to maintain synthesis of cartilage-type proteoglycans. This paper provides evidence that chondrocytes synthesize the appropriate proteoglycan matrix under TGF-β1 stimulation: (i) there is a coordinated increase in hyaluronic acid and proteoglycan monomer synthesis, (ii) link-stable proteoglycan aggregates are assembled, (iii) the hybrid chondroitin sulfate/ keratan sulfate monomeric species is synthesized, and (iv) there is an increase in protein core synthesis. Some variation in glycosylation patterns was observed when proteoglycans synthesized under TGF-β1 stimulation were compared to those synthesized under basal conditions. Thus comparing TGF-β1 to basal samples respectively, the monomers were larger (Kavon Sepharose CL-2B = 0.29 vs 0.41), the chondroitin sulfate chains were longer by ~3.5 kDa, the percentage of total glycosaminoglycan in keratan sulfate increased slightly from ~4% (basal) to ~6%, and the unsulfated disaccharide decreased from 28% (basal) to 12%. All of these variations are in the direction of a more anionic proteoglycan. Since the ability of proteoglycans to confer resiliency to the cartilage matrix is directly related to their anionic nature, these changes would presumably have a beneficial effect on tissue function.