Transmembrane Transport of Adenosine 5'-Triphosphate Using a Lipophilic Cholesteryl Derivative.
Transmembrane Transport of Adenosine 5'-Triphosphate Using a Lipophilic Cholesteryl Derivative.
复制标题
使用亲脂性胆固醇衍生物跨膜运输 5-三磷酸腺苷。
作者：
A. Kreimeyer;F. André;C. Gouyette;T. Huynh
脂质体中ATP的摄取是通过使用亲脂衍生物胆固醇氧羰基ATP实现的(1)。在pH梯度的帮助下观察到其水解导致囊泡内ATP的释放（见图），并通过31p核磁共振波谱进行监测。这是核苷5'-三磷酸第一次成功的跨膜转移。
The uptake of ATP in liposomes was achieved by using the lipophilic derivative cholesteryloxycarbonyl-ATP (1). Its hydrolysis leading to the release of ATP inside the vesicules (see scheme) was observed with the help of a pH gradient and monitored by 31 P NMR spectroscopy. This is the first successful transfer of a nucleoside 5'-triphosphate across a membrane.