The effect of local anesthetics on monocyte mCD14 and human leukocyte antigen-DR expression
The effect of local anesthetics on monocyte mCD14 and human leukocyte antigen-DR expression
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DOI：
10.1213/01.ane.0000104480.04856.93
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发表时间：
2004-04-01
影响因子：
5.7
通讯作者：
Shigematsu, A
中科院分区：
文献类型：
--
作者：
Kawasaki, T;Kawasaki, C;Shigematsu, A
已经证明，局部麻醉剂对免疫系统有几种影响。单核细胞和巨噬细胞是宿主对微生物感染反应的重要组成部分;然而，局部麻醉剂对单核细胞表面受体表达的影响仍不清楚。本研究旨在探讨局部麻醉药对单核细胞mCD 14和人类白细胞抗原（HLA）-DR表达以及脂多糖（LPS）或葡萄球菌肠毒素B（SE B）诱导的肿瘤坏死因子（TNF）-α产生的影响。从10名健康志愿者获得血液样品。通过酶联免疫吸附试验测定局部麻醉剂对LPS或SEB诱导的TNF-α产生的影响。加入不同剂量的局麻药后，用LPS（10 ng/mL）或SEB（10 μ g/mL）刺激血液4 h。采用双单克隆抗体染色和流式细胞术检测局麻药对单核细胞mCD 14和HLA-DR表达的影响。局部麻醉剂对人全血中LPS或SEB诱导的TNF-α产生无影响。局麻药呈剂量依赖性抑制单核细胞HLA-DR表达（P < 0.05），但对单核细胞mCD 14表达无影响。这项研究表明，局部麻醉剂抑制人类单核细胞表面的HLA-DR表达。
It has been demonstrated that local anesthetics have several effects on the immune system. Monocytes and macrophages are essential components of the host response to microbial infection; however, the effect of local anesthetics on monocyte surface receptor expression remains unclear. We designed this study to investigate the effects of local anesthetics on monocyte mCD14 and human leukocyte antigen (HLA)-DR expression and lipopolysaccharide (LPS)-induced or staphylococcal enterotoxin B (SEB)induced tumor necrosis factor (TNF)-alpha production. Blood samples were obtained from 10 healthy volunteers. The effects of local anesthetics on LPS- or SEB-induced TNF-alpha production were determined by using an enzyme-linked immunosorbent assay. After different doses of local anesthetics were added, the blood was stimulated with LPS (10 ng/mL) or SEB (10 mug/mL) for 4 h. The effects of local anesthetics on monocyte mCD14 and HLA-DR expression were measured by dual monoclonal antibody staining and flow cytometry. Local anesthetics showed no effect on LPS- or SEB-induced TNF-alpha production in human whole blood. Local anesthetics suppressed monocyte HLA-DR expression in a dose-dependent manner (P < 0.05) but had no effect on monocyte mCD14 expression. This study demonstrated that local anesthetics suppress HLA-DR expression on the surface of human monocytes.