Agricultural practices influence foliar endophytic communities in coffee plants of different varieties
Agricultural practices influence foliar endophytic communities in coffee plants of different varieties
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DOI：
10.1002/agg2.20476
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发表时间：
2024-03-01
期刊：
影响因子：
--
通讯作者：
Chaverri，Priscila
中科院分区：
文献类型：
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作者：
Castillo-Gonzalez，Humberto;Bloomberg，Joshua;Chaverri，Priscila
真菌内生菌是植物微生物组的关键组成部分，深刻影响其健康和健身。然而，关于这些微生物与其宿主之间的复杂相互作用，特别是在农业上重要的植物如咖啡的背景下，仍然存在无数的问题。为了弥合这一知识差距并提供一个全面的框架，本研究调查了农业实践如何塑造咖啡中叶平面内生菌的分类和功能多样性。对来自哥斯达黎加两个不同产区的咖啡植物叶片进行取样，确保代表各种咖啡品种（Obatá、Catuaí和Caturra）、农业管理方法（有机与常规）、阳光暴露制度（全日照/单作与自然遮荫/农林）和叶片发育阶段（新出现的无症状叶片与成熟叶片）。通过采用培养依赖性和独立技术（内部转录间隔区2核核糖体DNA元条形码）对真菌群落进行表征。结果表明，在成熟叶片和常规管理的植物内生菌的多样性更大，咖啡品种发挥不清楚的影响。令人惊讶的是，阳光照射的影响可以忽略不计。然而，数据强调了农林业和有机耕作的好处，这与减少假定的病原体和提高潜在互惠真菌的水平有关，促进了功能多样的社区。尽管植物微生物组可能在农业生产中发挥作用，但缺乏通过育种或管理来塑造内生菌群落的知识。这项研究的结果提供了一个框架，以了解植物和农业实践如何影响咖啡作物内内生菌的多样性。这些见解有望指导未来有效操纵咖啡微生物群落的努力。
Fungal endophytes are pivotal components of a plant's microbiome, profoundly impacting its health and fitness. Yet, myriad questions remain concerning the intricate interactions between these microorganisms and their hosts, particularly in the context of agriculturally important plants such asCoffea arabica. To bridge this knowledge gap and provide a comprehensive framework, this study investigated how farming practices shape the taxonomic and functional diversity of phylloplane endophytes in coffee. Coffee plant leaves from two distinct producing regions in Costa Rica were sampled, ensuring the representation of various coffee varieties (Obatá, Catuaí, and Caturra), agricultural management methods (organic vs. conventional), sun exposure regimes (full sunlight/monoculture vs. natural shade/agroforestry), and leaf developmental stages (newly emerged asymptomatic vs. mature leaves). Fungal communities were characterized by employing both culture‐dependent and independent techniques (internal transcribed spacer 2 nuclear ribosomal DNA metabarcoding). The results showed a greater diversity of endophytes in mature leaves and conventionally managed plants, with coffee variety exerting an unclear influence. The effect of sun exposure was surprisingly negligible. However, data emphasize the benefits of agroforestry and organic farming, which are linked to reduced putative pathogens and heightened levels of potentially mutualistic fungi, fostering functionally diverse communities. Despite the role that plant microbiomes might play in agricultural production, the knowledge to shape endophytic communities through breeding or management is lacking. The results from this study provide a framework to understand how both plant and agricultural practices influence endophyte diversity within coffee crops. These insights hold promise for guiding future efforts to manipulate coffee microbial communities effectively.