The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor.
The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor.
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大鼠 trk 原癌基因产物表现出慢神经生长因子受体的特性。
DOI：
10.1073/pnas.89.6.2374
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发表时间：
1992
影响因子：
11.1
通讯作者：
Shooter,EM
中科院分区：
文献类型：
--
作者：
Meakin,SO;Suter,U;Drinkwater,CC;Welcher,AA;Shooter,EM
两种不同的神经生长因子受体（NGFR）复合物存在于ngf反应细胞类型上；快速（fNGFR）和慢速（sNGFR） ngfr分别对应100 kDa和158 kDa。先前的研究表明，每个复合物都来源于一个单独的基因产物，并且sNGFR含有酪氨酸激酶活性。编码fNGFR的cDNA先前已被克隆。本研究从PC12细胞中分离出大鼠trk原癌基因cDNA，发现trk可与NGF结合，形成一个158 kDa的复合物。NGF-Trk相互作用的表征表明，Trk和NGF的解离比NGF和fNGFR的解离要慢。此外，ngf结合的Trk不会被胰蛋白酶消化破坏，而NGF-fNGFR复合物对胰蛋白酶消化很敏感。这些观察结果表明，在缺乏fNGFR的情况下表达的trk原癌基因产物可以将NGF与sNGFR的特性结合，该NGFR被鉴定为原代神经元和PC12细胞上的高亲和力NGFR。
Two distinct nerve growth factor receptor (NGFR) complexes are present on NGF-responsive cell types; these correspond to 100 kDa and 158 kDa for the fast (fNGFR) and the slow (sNGFR) NGFRs, respectively. Previous studies indicate that each complex is derived from a separate gene product and that the sNGFR contains tyrosine kinase activity. The cDNA encoding the fNGFR has previously been cloned. In this report, a rat trk protooncogene cDNA has been isolated from PC12 cells and Trk has been shown to bind NGF, generating a complex of 158 kDa. Characterization of NGF-Trk interactions indicates that Trk and NGF dissociate more slowly than do NGF and the fNGFR. Moreover, NGF-bound Trk is not destroyed by trypsin digestion whereas the NGF-fNGFR complex is sensitive to trypsin digestion. These observations suggest that the trk protooncogene product, expressed in the absence of the fNGFR, binds NGF with properties characteristic of the sNGFR, which was identified as the high-affinity NGFR on primary neurons and PC12 cells.