Mechanoreception in motile flagella of Chlamydomonas
Mechanoreception in motile flagella of Chlamydomonas
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DOI：
10.1038/ncb2214
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发表时间：
2011-05-11
影响因子：
21.3
通讯作者：
Yoshimura, Kenjiro
中科院分区：
文献类型：
--
作者：
Fujiu, Kenta;Nakayama, Yoshitaka;Yoshimura, Kenjiro
纤毛虫和鞭毛虫在碰撞时通过产生机械感受器电位暂时向后游。虽然这种潜力已被证明是与草履虫纤毛，机械感受器的分子实体仍然未知。在这里，我们表明，衣原体细胞表达TRP11，TRP（瞬时受体电位）亚家族V的成员，在近端区域的鞭毛，和抑制TRP11表达的结果在损失的避免反应。结果表明，衣原体鞭毛表现出mechanosensitivity，尽管恒定的运动，通过本地化的mechanoreceptor在近端区域，其中主动弯曲受到限制。
Ciliates and flagellates temporarily swim backwards on collision by generating a mechanoreceptor potential. Although this potential has been shown to be associated with cilia in Paramecium, the molecular entity of the mechanoreceptor has remained unknown. Here we show that Chlamydomonas cells express TRP11, a member of the TRP (transient receptor potential) subfamily V, in the proximal region of the flagella, and that suppression of TRP11 expression results in loss of the avoiding reaction. The results indicate that Chlamydomonas flagella exhibit mechanosensitivity, despite constant motility, by localizing the mechanoreceptor in the proximal region, where active bending is restricted.